刺萼龙葵对二十八星瓢虫的化学防御机制研究

Solanum rostratum is a worldwide malignant invasive weed, causing serious harm to the ecological environment and biodiversity of the northern part of China. This plant is difficult to control in addition to its own strong propagation, rapid growth and extensive ecological adaptability, and also its successful chemical defense to phytophagous insects. Previous research has shown that it has significant chemical defense characteristics to the solanaceae important pest-Henosepilachna vigintioctopunctata, however, the chemical defense mechanism.is unclear. The project intends to conduct the thorough research to the chemical defense of Solanum rostratum by non-feeding and feeding treatment of Henosepilachna vigintioctopunctata. Extraction, separation and structural identification the secondary metabolites of Solanum rostratum to find its constitutive and induced chemical defensive composition, and compare the difference and connection between the two chemical defense. And through the anti-insect activity of function verification, determine the effect of defensive composition on Henosepilachna vigintioctopunctata, in order to clarify the chemical defense rule and mechanism of action from the molecular level. The results of the study will help to reveal the invasion mechanism from the perspective of chemical defense, and is expected to obtain the active substance with the application prospect of plant insecticide, which can provide a scientific basis for the development and utilization of Solanum rostratum, and also lay the foundation for comprehensive prevention and control of Henosepilachna vigintioctopunctata.

刺萼龙葵是一种世界性的恶性入侵杂草，对我国北方地区的生态环境和生物多样性造成了严重危害。该植物之所以难以彻底防治，除了因为它本身具有繁殖量大、生长发育快和广泛的生态适应性外，还与其对植食性昆虫成功的化学防御有着密切的关系。前期研究表明刺萼龙葵对茄科重要害虫-二十八星瓢虫具有显著的化学防御特性，但其化学防御机制尚不明确。本项目拟通过二十八星瓢虫非取食和取食诱导处理的方法深入刺萼龙葵的化学防御机制，对其次生代谢产物进行系统的提取分离和结构鉴定，发现其组成型和诱导型化学防御成分，比较两者之间的差异和联系，并对所分离得到的防御成分进行抗二十八星瓢虫活性的功能验证，以期从分子水平上阐明其化学防御规律及作用机制。研究结果将有助于从化学防御角度揭示其入侵机制，并期望获得具有植物源抗虫农药应用前景的先导活性物质，最终将为刺萼龙葵的开发利用提供科学依据，同时也为二十八星瓢虫的综合防治奠定基础。

刺萼龙葵是一种世界性的恶性入侵杂草，对我国北方地区的生态环境和生物多样性造成了严重危害。该植物之所以难以彻底防治，除了因为它本身具有繁殖量大、生长发育快和广泛的生态适应性外，还与其对植食性昆虫成功的化学防御有着密切的关系。本项目通过二十八星瓢虫非取食和取食诱导处理的方法深入刺萼龙葵的化学防御机制，对其次生代谢产物进行系统的提取分离和结构鉴定，最终发现其组成型化学防御成分8个和诱导型化学防御成分2个，比较了两者之间的差异和联系，并对所分离得到的防御成分进行抗二十八星瓢虫活性的功能验证。研究结果有助于从化学防御角度揭示其入侵机制，并期望获得具有植物源抗虫农药应用前景的先导活性物质，最终将为刺萼龙葵的开发利用提供科学依据，同时也为二十八星瓢虫的综合防治奠定基础。