多目标同时检测的DNA分子逻辑门技术研究

Nowadays, there is a serious problem in the field of computer. That is the integration level of traditional computers' processor which based on semiconductor material and integrated circuits have been approached its theoretic limitation, as a result, this situation greatly restrains further progress of the computer technology. So, scientists are trying their best to constructing the novel computers. The DNA computer is regarded as one of which and have already to be an important research direction in this domain. As we all know, logic operation is the cornerstone for the construction of computers. A range of systems having logic operation function applying DNA molecules have been designed and constructed in recent years. However, almost all of these systems employ optical or colorometric signals as their outputs, which in some extent limits their application in computers. Inherited the previous works of other scientists, this project will develop the novel molecular switch technique for the detection of the characteristic gene sequences of common food-borne pathogenic bacteria with electrochemical technology. And then, DNA molecular logic gates will be established to realize the goal to detection several different DNA molecules at same time, fastly and intelligently. Furthermore, to establish the DNA logic gates having the basic logic operation function, which will pave a path for the development of DNA computer.

目前，以半导体材料和集成电路为基础的计算机技术由于其集成度已接近理论极限，限制了其进一步发展。科学家们正在积极开发建立新型计算机，DNA计算机被认为是未来计算机科学发展的重要方向之一。众所周知，计算机的构建是以逻辑运算为基础。科学家们已经研究出一些具有逻辑运算功能的DNA体系，然而大多数此类体系都是基于光学信号识别或检测系统，这种信号识别系统在用于新型DNA计算机开发上具有一定的局限性。本项目拟在继承前人工作的基础上，以电化学检测技术为手段，发展针对常见食源性致病菌特征基因序列分析检测的新型分子开关技术；构建基于电化学检测技术的DNA分子逻辑门体系，实现对多目标DNA分子的同时、快速、智能检测；进一步构建具有基本逻辑运算功能的DNA逻辑门体系，为DNA计算机的研究和发展做一些基础性的探索和铺垫工作。

目前，以半导体材料和集成电路为基础的计算机技术由于其集成度已接近理论极限，限制了其进一步发展。科学家们正在积极开发建立新型计算机，DNA计算机被认为是未来计算机科学发展的重要方向之一。众所周知，计算机的构建是以逻辑运算为基础。本项目在继承前人工作的基础上，以电化学检测技术为手段，发展针对DNA序列分析检测的新型分子开关技术；构建基于电化学检测技术的DNA分子逻辑门体系，实现对多目标DNA分子的同时、快速、智能检测；进一步构建具有基本逻辑运算功能的DNA逻辑门体系，为DNA计算机的研究和发展做一些基础性的探索和铺垫工作。.本项目研究内容包括以下几个方面：.1.构建了一系列针对不同目标DNA分子的新型分子开关及逻辑门系统，实现对目标DNA的快速、灵敏检测。.2.通过实验验证了所构建的基于电化学检测技术的新型 DNA 分子逻辑门体系具有很好的逻辑判断功能，即可以实现多目标DNA分子的同时检测，具有智能区分识别的功能。.3.进一步研究建立了几种具有基本逻辑运算功能的DNA 分子逻辑门体系，包括两种半加器和一种半减器，验证了DNA逻辑运算器的可行性，为基于DNA分子的计算机研究与开发做了最基本的探索性工作。