仿生抗体类似物材料制备及性能研究

Poly (amidoamine) (PAMAM) dendrimers with sphere shape (generation 1-5) and fan shape (generation 1-3) were synthesized..The self-assembly ultrathin films with PAMAM dendrimers (carboxylate salts) as polyanions and NDR or DR (Nitrocontaining diazoresin or Diazoresin) as polycations were fabricated and studied. It proves that these unique compounds could recognize and combine with other molecules via electrostatic or H-bond interactions..The peptides of Gly-Asp, Gly-Asp-Gly-Asp, Gly-Asp-(Gly-Asp)2, Gly-Glu and Gly-Glu-(Gly-Glu)2 were prepared and introduced at the periphery of G3.5 PAMAM dendrimers, respectively. These modified dendrimers show bioactivity. They could obviously decrease the reduction rate of FeIII-cyt C by ascorbic acid, especially, the dendrimers with Gly-Asp-(Gly-Asp)2 and Gly-Glu-(Gly-Glu)2 on their surface. Both of the association constants of G3.5-Gly-Asp-(Gly-Asp)2 and G3.5Gly-Glu-(Gly-Glu)2 with cytochrome C are 3.2ⅹ105 and the conformations of them are quite similar to β-sheet by molecular simulation.It has been found that (1) higher generation dendrimers show better affinity with cytochrome C. (2) The peptides with longer chain demonstrate better affinity with cytochrome C. (3) The dendrimers with more modified exterior groups exhibit higher affinity with cytochrome C. (4) Compared with sphere shape dendrimers, the molecules coupled with fan shape dendrimers show stronger affinity with cytochrome C. Dendrimer chemistry and application is an attractive and active study field. It is believed that the application of dendrimers in drug delivery will be a breakthrough. This project is of significance for it establishes the foundation of the study on the applications of dendrimers in biochemistry and medicine..

本项目以不同结构，不同代数的树枝状大分子为起始物，经化学?饰或自组装，使其具备识别蛋白质表面的功能。最终得到纳米级的，分子结构、分子量可调控的新型仿生抗体材料。预计它识别能力强，性能稳定，生物相似性好，有重要的科学研究价值和应用前景。.