新型多功能中药溶致液晶载药系统的构建及其抗肿瘤作用研究

In this research, based on the phase behavior of the pseudo-ternary system of lipids/water/oil, the pH response and photosensitive in situ forming bufalin loaded lyotropic liquid crystalline (LLC) drug carrier system and multifunctional targeting bufalin loaded LLC nanoparticles will be designed and prepared. Through the study on the characterization of drug concentration effect on the structure and lattice parameters of the liquid crystal phase, the interactions between drugs and liquid crystal nano carrier matrix, and the loading and releasing mechanism of drug active molecules in the lyotropic liquid crystalline drug carrier will be revealed. The mechanism and therapeutic effect of bufalin new dosage in the treatment of cancer based on lyotropic liquid crystalline drug carrier will be obtained from the molecules, cells to the tissue levels. The in vivo safety and biological stability of bufalin new dosage will also be studied. It will be hoped that some certain original achievements for the treatment of tumor with traditional Chinese medicine will be improved by the application of nanotechnology.

基于脂质/水/油拟三元体系相行为的研究，设计和构建具有pH响应、光敏性等原位成型蟾毒灵溶致液晶载药系统及多功能靶向蟾毒灵溶致液晶纳米粒；通过研究表征药物浓度对液晶相结构和晶格参数的影响，理解药物与液晶纳米载体基质的相互作用，探讨药物活性分子在溶致液晶药物载体中的载药机制和释放机理；在分子、细胞、组织水平上揭示蟾毒灵新剂型肿瘤治疗中的作用机制和治疗效果，了解其体内的安全性及生物稳定性；在纳米技术改善中药治疗肿瘤现状的应用基础研究方面取得一定的原创性成果。

聚焦药物传递的靶向、控释两大关键问题，基于脂质/水/油拟三元体系相行为的研究，为蟾毒灵、鸦胆子油等传统抗肿瘤中药设计和构建具有pH响应、叶酸及RGD肽靶向等功能型溶致液晶载药系统；通过研究表征药物浓度对液晶相结构和晶格参数的影响，理解药物与液晶纳米载体基质的相互作用，探讨药物活性分子在溶致液晶药物载体中的载药机制和释放机理，优化靶向及控释纳米载药系统；在分子、细胞、组织水平上揭示蟾毒灵等新剂型肿瘤治疗中的作用机制和治疗效果，了解其体内的安全性及生物稳定性；实现抗肿瘤中药主动靶向控释剂型的构建，在纳米技术改善中药治疗肿瘤现状的应用基础研究方面取得一定的原创性成果。