食品和药品基体中未知化学物质的分离和分析方法研究

Adulteration，counterfeit, food poisoning and food terrorism are the critical problematic factors of food and drug safety. Nontargeted screening for harmful chemicals in food and drugs is always a big challenge in the field of analytical chemistry, due to not only the unknown target substance but the complex sample matrix. To meet the demand of monitoring and ensuring food and drug safety, this proposal aims to develop a comprehensive nontargeted screening methodology of chemical hazardous in food and drugs based on the technologies of food chemistry, medicinal chemistry, instrumental analysis, mathematical statistics as well as computer science. This study includes 3 parts of contents: establishing a high-capacity mass spectra library of chemicals using ultra-performance liquid chromatography coupled with high-resolution mass spectrometry (UPLC-HRMS), developing the sample pretreatment strategy for nontargeted chemical screening in food and drug matrix, as well as a data process software of the UPLC-HRMS chromatograms of real samples. Then, a rapid and effective pretreatment and analytical procedure is established to identify the unknown harmful chemicals in food and drugs. This study will provide scientific support of food and drug security system.

化学掺假、冒牌、食物中毒、食物恐怖等问题是现阶段我国食品药品安全的主要问题。食品药品等复杂基体中未知化学物质的筛查是卫生检验领域的难点之一。本项目将在已有研究的基础上，以食品药品安全科技需求为导向，以相关基体中未知化学物质分离分析为目标，利用食品化学、药物化学、仪器分析、数理统计、计算机科学等技术，从分离、分析和数据处理等多个角度入手，构建大容量的有毒有害化学物质的液相色谱高分辨质谱数据库，建立食品药品化学危害物高回收前处理方法，开发未知样品质谱数据处理软件。建成一整套快速、高效的危害物分离分析方法体系，形成易操作的、标准、统一的食品药品中未知化学物质非定向检测流程，为食品药品安全保障提供技术支持。

化学掺假、冒牌、食物中毒、食物恐怖等问题是现阶段我国食品药品安全的主要问题。食品药品等复杂基体中未知化学物质的筛查是卫生检验领域的难点之一。非定向筛查技术可为处理相关安全事件和监管食品安全提供新的解决方案。本项目主要围绕食品和药品中化学危害因子非定向筛查样品前处理及全回收、不同层次数据库构建和筛查技术平台开展研究，设计、制备了一系列新型的样品前处理材料，构建了5000余种危害因子的液相色谱高分辨质谱标准数据库，汇总了典型危害因子的质谱裂解规律，采集获得了9类食品药品的本源组成质谱数据。完成了化学危害物数据收集系统和非靶向筛查系统的开发，可实现五家公司高分辨质谱数据解析与转换，系统测试效果良好。项目成果成功用于食物中毒事件、保健品掺伪等未知化学物质的筛查，撰写了食品药品中化学危害因子非靶向筛查标准操作规程。以上研究可为食品药品安全保障提供技术支持。