从粘膜免疫HMGB1通路研究益气散结法在膀胱癌卡介苗灌注中减毒增效的机制

Although Bacillus Calmette-Guerin ( BCG ) perfusion treating bladder cancer has a good clinical efficacy, the side effects is obviously. We developed a Replenishing qi and dissipating mass formula which called Qi Ci Leng Decoction (QCLD), Astragalus root as the major component, to attenuate the side effects and increase efficacy. However, its mechanism is still unkown. Synergistic effects and mechanisms by observation of QCLD combined with BCG in the treatment of bladder cancer in vivo and in vitro. Our research would help to further clarify the molecular mechanism of QCLD combined with BCG in the treatment of bladder cancer attenuated synergies.

卡介苗（BCG）灌注治疗膀胱癌临床疗效确切，但其副作用明显。我们应用益气散结法自拟以黄芪为君药的芪慈棱汤对BCG膀胱灌注能起到明显的减毒增效作用,但其机制尚不明了。本研究拟通过体内和体外实验,观察芪慈棱汤联合BCG治疗膀胱癌的减毒增效作用及部分机制，研究结果将有助于为进一步阐明芪慈棱汤联合BCG治疗膀胱癌减毒增效作用的分子机制奠定基础。

卡介苗（BCG）灌注治疗膀胱癌临床疗效确切，但其副作用明显。我们应用益气散结法自拟以黄芪为君药的芪慈棱汤对BCG膀胱灌注能起到明显的减毒增效作用,但其机制尚不明了。本课题组通过1年的研究，在原有进度安排计划下，顺利完成了各项实验指标的检测，达到了预定目标。我们通过体内、体外实验对芪慈棱汤对BCG的减毒增效作用进行了初步的研究，初步实验结果认为芪慈棱汤对BCG处理的膀胱癌细胞能直接抑制膀胱癌细胞体外增殖；能促进膀胱癌细胞的凋亡；调控BCG处理的膀胱癌细胞的细胞周期，使细胞生长停止在G0-G1期，阻止细胞进入S期进行DNA复制；另外芪慈棱汤及BCG作用使大鼠膀胱肿瘤生长缓慢，肿瘤直径增长较小，肿瘤细胞增殖抗原Ki-67、PCNA在大鼠膀胱肿瘤中有明显表达。