人急性髓系白血病相关逆转录病毒全长基因组结构研究

: Human leukemia is a common hematopoietic malignant tumor, and its etiology and mechanism remain unknown so far.Our previous studies demonstrated that human leukemic cells contain novel retrovirus. In this study, we successfully isolated and cloned the full-length genome of a novel human retrovirus from primary leukemic cells from leukemia patients using PCR, RT-PCR, RACE, DNA molecular cloning, bioinformatics, transfection assay and other methods. Sequence analysis showed that: the proviral DNA genome consists of 8824 bp, and its genomic structure is5`-LTR-UTR-GAG-POL-ENV-3`LTR; the viral RNA genome is 8400 bp in size, and its genomic structure is 5`-RU5-UTR-GAG-POL-ENV-U3R-POLYA-3`. Alignment analysis and transfection results suggest that this novel retrovirus may belong to type C oncogenic retrovirus. Isolation and identification of the novel retrovirus genome might be of great importance to elucidate the etiology and mechanism of human leukemia.

.1996年我们在国际上首次报导大多数人急性白血病细胞中含有可能与白血病病因相关的新湍孀级荆罱颐且殉晒Φ毓菇撕《净蚱蔚娜思毙运柘蛋籽∠赴鹀DNA文库。狙芯?应用cDNA末断快.速扩增技术和基因组文库构建技术对新发现的病毒全长基因组cDNA颓安《净?组DNA进行分离和克隆，然后通过核酸序列测定和计算机系统研究病毒基因榻峁埂?