蜀葵细胞自噬作用对小孢子发育的影响

Autophagy plays important roles in the development of microspores in plant.However, the specific physiological function and regulating mechanism of autophagy in the development of microspores is unclear. Althaea Rosea (L.) Cavan is a very important medicinal plant. It is a strong ornamental with beautiful flowers and suitable for large area planting.So our research will focus on the impact of autophagy to the development of microspores in Althaea rosea (L.). First,the transmission electron microscope will be used to observe the ultrastructure of the cells, the phenomenon of autophagy, the relationship between autophagosome and cytoplasmic channel and the structrural change of tapetum layer. And then we will use fluorescence staining and confocal laser technology for further observation about the phenomenon of autophagy and the cellular location of autophagosome in the development of microspores. On these basis, the nuclear magnetic resonance, spectrophotometer, TTC method, polyacrylamide gel electrophoresis methods will be used to analyze the alkaline phosphatase, choline phosphate, pollen viability, peroxidase, cytochrome oxidase and other indicators. Finally,We will consider all the data together to analyze the influence of autophagy in the development of microspores, in which will provide basic data for the further research about regulation mechanism of autophagy in the microspore development in plants.

自噬在植物小孢子发育过程中发挥重要作用，但其详细的生理功能和调控机制还不清楚。蜀葵（Althaea Rosea (L.) Cavan）是重要的中药材之一，且因花大色艳而观赏性较强，适合大面积种植。本研究以蜀葵为材料，首先采用透射电子显微镜方法观测细胞超微结构、自噬现象、自噬体与胞质通道的联系及绒毡层的结构变化，进而采用荧光染色、激光共聚焦显微镜技术进一步观测蜀葵小孢子发育过程中细胞内的自噬现象及自噬体的细胞定位。以此为基础，采用分光光度计、核磁共振、四唑（TTC）法、聚丙烯酰胺凝胶电泳等方法，分析细胞的碱性磷酸酶、磷酸胆碱、花粉活力、过氧化物酶、细胞色素氧化酶等指标，以此判断细胞自噬对蜀葵小孢子发育的影响，为进一步开展自噬对植物小孢子发育的调控机制研究提供基础数据。