CNTF和GAP-43对大鼠视网膜神经节细胞的影响
基本信息
结项摘要
研究表明睫状神经生长因子（CNTF）和生长相关蛋白-43（GAP-43）能够促进视神经的再生。最近，我们发现CNTF及其受体mRNA在视神经损伤及修复的过程中表达明显增加，玻璃体腔内注射适量CNTF能促进视神经的再生，并成功地建立了视神经-坐骨神经吻合后视神经再生模型。本项目拟在以上研究基础之上，进一步探讨CNTF和GAP-43对大鼠视网膜神经节细胞（RGC）的影响。利用RT-PCR技术，克隆CNTF和GAP-43 cDNA，构建CNTF和GAP-43的腺相关病毒质粒；通过病毒感染，检测CNTF和GAP-43在大鼠体内（in vivo）和体外（in vitro）的RGC中表达水平和持续时间、分析CNTF和GAP-43过表达是否能够促进视神经的再生和保护RGC，为视网膜及视神经疾病的基因治疗提供理论依据。
项目摘要
项目成果
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数据更新时间：2023-05-31
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