单胺类神经递质及其代谢物活体原位采样、在线印迹富集、实时检测新方法研究

Monoamine neurotransmitter is one of the most important neural transmission medium with broad biological activities. Some nervous system diseases such as depression are always associated with the abnormal level of monoamine neurotransmitter. Therefore, in vivo monitoring techniques for the determination of monoamine neurotransmitters and their metabolites in living organisms have become advanced topics in nerve biochemistry and analytical chemistry. In this project, in vivo microdialysis sampling technique,online imprinted microextraction sample preconcentration and separation technique coupled with the rapid and efficient chromatographic and spectroscopic detection techniques are developed. It mainly involve several aspects including developing monoamine neurotransmitters molecularly imprinted microextraction medium, designing an interface for coupling of in vivo microdialysis sampling and the simultaneously imprinted microextraction, building the real-time dynamic monitoring device for monoamine neurotransmitters, exploring a new online analytical method for determination of the monoamine neurotransmitters and their metabolites in living organisms and its application in neural biochemical mechanism of depression, in emotion and memory processes study. This research work will provide scientific methods for the causes, diagnosis and treatment of depression, and further promote the physiological, pathological and pharmacological research.It will provide the key methods and techniques for the research on emotion and memory processes from the molecular level.

单胺类神经递质是一类具有广泛生物学活性的物质，抑郁症等神经系统疾病与单胺类神经递质的异常有关。因此，生物活体中单胺类神经递质及其代谢产物原位实时在线分析检测技术及其方法研究成为神经生物化学及分析化学的前沿课题。本项目集成微透析活体采样技术、印迹微萃取在线分离富集技术及快速高效的色谱及光谱检测技术于一体，研制单胺类神经递质分子印迹微萃取介质；设计生物活体中单胺类神经递质微透析活体原位采样-印迹微萃取联用接口，构建实时动态监测装置；研究生物活体中单胺类神经递质及其代谢产物实时在线分析检测新方法；探索实时在线分析检测新技术在抑郁症神经生化机制、情感和记忆过程研究中的应用。研究成果将为抑郁症等疾病的产生及诊疗研究提供科学方法手段，将促进生理学、病理学及药理学的研究，为从分子水平上开展情感与记忆过程研究提供关键方法与技术。

单胺类神经递质是一类具有广泛生物学活性的物质，抑郁症等神经系统疾病与单胺类神经递质的异常有关。因此，生物活体中单胺类神经递质及其代谢产物的在线分析检测技术及其方法研究成为神经生物化学及分析化学的前沿课题。本项目集成在线分离富集样品前处理技术及快速高效的色谱及光谱检测技术于一体，以单胺类神经递质及代谢物为研究对象，开展了复杂生物样品中痕量单胺类神经递质及其代谢物在线分析方法研究。. 设计研制了2种分别用于单胺类神经递质及其酸性代谢物在线分离富集的选择性高、稳定性好的功能萃取材料，发展了2种微固相萃取固载技术；研制了1套流动电解装置，发展了一步法电解石墨制备高纯度羧基功能化碳纳米片的新技术，制备了1种对5-羟基色氨酸敏感的修饰电极材料；构建了2套生物样品中单胺类神经递质及其酸性代谢物在线微萃取-色谱联用分析系统；建立了2种测定生物样品中痕量单胺类神经递质及其酸性代谢物的色谱联用在线分析新方法、1种检测大鼠脑组织样品中5-羟基色氨酸的高灵敏电化学分析新方法；探索了新方法在实际样品分析中的应用。同时结合各种新型样品前处理介质分别开展了分子印迹微萃取-分步聚焦/超高效液相色谱在线联用系统研制及其应用、配位印迹荧光传感器研制及其应用等研究。. 研究成果在Journal of Chromatography A、Analytica Chimica Acta等重要学术刊物上发表论文15篇，其中SCI收录论文13篇，核心刊物2篇，获得授权发明专利2件。在国内外学术会议上作邀请报告5次。培养博士生3名、硕士生5名、本科生3名。研究成果将为抑郁症等疾病的产生及诊疗研究提供科学方法手段，将促进生理学、病理学及药理学的研究，为从分子水平上开展情感与记忆过程研究提供关键方法与技术。