针刺对高脂血症心脏的保护作用及其机制

Along with lifestyle changes, hyperlipidemia incidence increases rapidly, the current national lipid abnormalities in patients is more than 16 million。High blood lipids can cause hypertension and atherosclerosis, induce cardiovascular and cerebrovascular disease.Lowering lipid effectivly and protecting cerebrovascular and cardiovascular are very important.Common lipid-lowering drugs have side effects such as liver and kidney damage, and on cardiovascular target organ protection is unsatisfactory. Previous studies found that acupuncture therapy effect is clear, no toxic side effect, and can relieve clinical symptoms such as chest palpitations. But whether acupuncture can protect heart of the hyperlipidemia patients and its mechanism is still not clear. We use cardiac ultrasound, VG staining to observe heart proctecting effect of long term acupuncture on ApoE Mice, and application of ELISA, immunohistochemistry, Western-blot, RT-PCR, observe the effects of acupuncture on ApoE Mice peripheral blood ANGII, ET1, NO levels as well as the heart ATIR, AT2R, ETAR, ETBR protein expression influence, use RRA check the combination of ANGII, ET1 with their recipients,discuss acupuncture protecting hyperlipidemia cardiovascular damage mechanism.This thesis will reveal the mechanism of cardiac and vascular protection by acupuncture on hyperlipidemia patients , and provide theoretical basis for clinical use.

随着生活方式改变，高血脂发病迅猛增加，目前全国血脂异常患者超过1.6亿。高血脂可诱发心脑血管病，有效降脂保护心脑血管具有重要意义。常用降脂药物有损害肝肾功能等副作用，且对心脏等靶器官保护不尽如人意。以往研究发现针刺降脂疗效明确，无毒副作用，且能减轻胸闷心悸等临床症状。但针刺能否保护高脂血症心脏及其机制尚不明确。我们应用心脏超声、VG染色等观察针刺对ApoE基因缺陷小鼠心脏的保护作用，同时应用ELISA、免疫组化、Western-blot、RT-PCR和放射性配体受体结合实验等手段，观察针刺对APoE基因缺陷小鼠外周血ANGII、ET1、NO水平以及对心脏ATIR、AT2R、ETAR、ETBR等蛋白表达的影响和对ANGII、 ET1与其受体结合率的影响，探讨其心脏保护作用机理。本课题的完成将揭示针刺对高脂血症患者心脏保护作用机制，为针刺治疗高脂血症临床运用提供理论依据。

随着现代人们生活方式的改变，高血脂症患者增多，据推算目前全国血脂异常患者已超过1.6亿。高血脂症是诱发心脑血管病的高危因素，故有效降脂，对保护心脑血管具有重要意义。现今常用降脂药物有损害肝肾功能等副作用，且对心脏等靶器官保护不尽如人意。根据以往研究发现针刺降脂疗效明确，无毒副作用，且能减轻胸闷心悸等临床症状。但针刺能否通过对脂质过氧化的一系列影响，保护高脂血症心脏尚不明确。本课题中应用了心脏超声、VG染色等观察针刺对ApoE基因缺陷小鼠(ApoE gene knock-out mice, ApoE-/- mice)心脏的保护作用，同时应用ELISA、Western-blot等手段，观察针刺对APoE-/-小鼠外周血AngⅡ、ET1、IL-17水平以及对心脏AT1R、ETAR等蛋白表达的影响，探讨其心脏保护作用机理。通过ELISA、血生化及免疫组化等，检测外周血血清胆固醇（TC）变化、白介素-17（IL-17）和NO波动，脂肪肝肝内MDA和SOD、IL-17改变，探索针刺对高血脂症及其对脂质过氧化一系列反应的影响。研究结果表明：①针刺丰隆及内关穴可降低心重体重比；减少ApoE-/-小鼠心肌组织中胶原纤维；及减轻ApoE-/-小鼠心室前后壁增厚程度。②针刺内关和丰隆穴可降低外周血AngⅡ、ET-1水平；抑制心脏组织中AT1R、ETAR表达，发挥对心脏的保护作用。③针刺内关和丰隆穴可使ApoE-/-小鼠血脂升高幅度明显降低；可降低血清IL-17含量；可改善ApoE-/-小鼠肝组织内结构形态及降低脂质含量；还可降低ApoE-/-小鼠肝组织内IL-17的表达；针刺内关和丰隆穴及辛伐他汀灌胃皆可升高外周血NO含量；但对减缓ApoE-/-小鼠脂质过氧化的效果并不明显。本课题的完成探索了针刺对高脂血症的改变及对心脏保护作用的部分机制，为针刺治疗高脂血症临床运用提供一定理论依据。