毛竹LTR反转录转座子响应环境变化的分子机理

LTR retroelements are the main components of moso bamboo gene expression regulation network and medium of moso bamboo responding to the environmental changes. Based on the previous research advances, the project plans to analyze the transposition behavior and expression pattern of moso bamboo LTR retroelements during stress treatment, investigate the dynamic change of DNA methylation of moso bamboo genome, and transcription level of non-coding RNA and mRNA, dissect the interaction model of LTR retroelements, non-coding RNA, and DNA methylation, explore the means of of moso bamboo LTR retroelements responding to environmental changes. The moso bamboo active LTR retroelements would be transformed into rice. Research on the influence of DNA methylation and stress treatment of transposition frequency of moso bamboo active LTR retroelements would be focused. The impact of transposition of moso bamboo active LTR retroelements on host gene expression would also be analyzed. The moso bamboo active LTR retroelements would also be transiently transformed into moso bamboo seedlings. The transcription level of siRNAs and lncRNAs encoded by moso bamboo active LTR retroelements and the genes targeted by siRNA and lncRNA would be analyzed. The mechanism of LTR retroelements regulating the host gene expression by non-coding RNA would be explained. In all, if the project would be carried out successfully, molecular mechanism of moso bamboo LTR retroelements responding to environmental changes would be displayed with multi-dimension, and the research level of transposable elements would be advanced.

LTR反转录转座子是毛竹基因表达调控网络的重要组成部分，是毛竹响应环境变化的重要媒介。本项目组计划在以往的工作基础上，分析毛竹LTR反转录转座子在逆境中的转座行为和表达模式，检测毛竹基因组甲基化水平的动态变化，非编码RNA和mRNA转录水平的变化，剖析反转录转座子，非编码RNA，DNA甲基化三者互作机制，探索反转录转座子对环境变化的响应途径；将毛竹活性LTR反转录转座子，遗传转化到水稻，分析和阐明胁迫处理和DNA甲基化水平变化对LTR反转录转座子转座频率的影响，以及转座子跳跃对水稻基因表达的影响；将活性LTR反转录转座子瞬时转化毛竹，分析转座子编码的siRNA或lncRNA转录水平的变化，以及调控的靶基因表达水平的变化，阐明反转录转座子通过非编码RNA调控宿主基因表达的机制。总之，本课题如能成功实施，将在多维度剖析LTR反转录转座子对环境变化的响应机制，提升转座子研究的理论水平。

LTR反转录转座子是毛竹基因表达调控网络的重要组成部分，是毛竹响应环境变化的重要媒介。本项目组分析了毛竹LTR反转录转座子在毛竹非生物胁迫、形态变异和快速拔节过程中的转座行为和表达模式，检测毛竹基因组甲基化水平的动态变化，非编码RNA和mRNA转录水平的变化，剖析反转录转座子，非编码RNA，DNA甲基化三者互作机制，探索反转录转座子对环境变化的响应途径；将毛竹活性LTR反转录转座子Ph-LTR1、PHRE2和PHRE11遗传转化到模式植物和酵母中，分析和阐明了胁迫处理对LTR反转录转座子转座频率的影响，以及转座子跳跃对宿主基因表达的影响；将活性LTR反转录转座子瞬时转化毛竹实生苗，分析转座子编码的circRNA、lncRNA和非典型转录本的转录水平变化，以及调控的靶基因表达水平的变化，阐明了反转录转座子通过非编码RNA调控宿主基因表达的机制。总之，本课题剖析了LTR反转录转座子对环境变化的响应机制，提升转座子研究的理论水平。.在本项目实施和完成过程中，共发表文章25篇文章，其中SCI收录12篇，一级期刊4篇，核心期刊9篇。参加3次学术会议，9位硕士研究生毕业，1位博士生毕业，两个博士后出站，超额完成原先预定的研究目标。