纳米孔单分子医疗诊断器件的关键技术研究

The project intends to develop a nanopore single-molecule detection device for medical diagnosis. This device is based on the nanopore technology, the principle of which is just like Coulter Counter. When the bio-molecules with charges could be driven by the electrical force to pass through the nanopore and could physically block the pore to produce measurable changes in ionic currents. A transient electrical current changing is used to detect the bio-molecules in the solution.. We propose to creat the nano cage which involve the top-membrane with the small nanopore ,the bottom-membrane with the bigger nanopore and the follow chamber. Based on this cage, we could distinguish the immunocomplex caused by the specific binding of antibody and antigen under the positive and negative potential driving. This project will also focus on the modeling and analysis of the impact between the fludic dynamics and protein molecules transport behaviors in the nanopore channel, which is expected to establish the influencing laws of the protein molecules and other factors。

纳米孔单分子医疗诊断器件是实现精准医疗诊断的最可行的技术方案之一，可实现抗原-抗体的特异性结合检测，从而有望精准的判断出致病因素，具备进行亚群分类的能力，并有助于对个体进行差异性治疗，提高人们健康医疗水平。. 本项目拟研制出具有纳米笼结构、并可控尺寸的纳米孔单分子医疗诊断器件，进而得以实现抗原分子、抗原－抗体结合物的选择性通过，并利用乒乓原理（正反向电压驱动），实现生物分子的碰撞信号和过孔信号的有效辨识，以期实现抗原－抗体结合物的精准性辨识，从而实现疾病的精确诊断；同时将致力于蛋白质分子在纳通道内输运机理的研究，以期了解各因素对蛋白质分子在纳通道内输运行为的影响规律。

纳米孔单分子医疗诊断器件是实现精准医疗诊断的最可行的技术方案之一。.本项目优化工艺，提高了纳米孔制备的可控程度；进行了具有纳米笼结构的纳米孔单分子医疗诊断器件制备与集成，并实现了生物分子的碰撞信号和过孔信号的有效辨识和抗原－抗体结合物的辨识；同时开展了蛋白质分子在纳通道内输运机理的研究，研究发现蛋白质在电场作用下会发生转动，同时在大场强的作用下会发生解折叠现象,为实验现象分析提供思路和依据。