热休克蛋白过表达抵抗心肌应激损伤的分子机制

To research the relationship between high sudden death and the reduction of heat shock proteins (Hsps) expression in the heart during heat stress, especially at the beginning of heat stress, and prevent the possibility of stress damage and reduce the mortality of the stressing animals, Hsps will be over-expressed seperately in the myocardial cells of broilers in vivo and in vitro under the air-controled lab and normal feeding condition by means of Aspirin treatment. The variations of inducible and consititutive hsps mRNA transcriptions and their corresponding Hsps expressions, the pathological lesions of the heat stressed myocardial cells, the relationship between stress damage and Hsps distribution and expression, Hsps protection and their signal transduction in the heat stressed myocardial cells during heat stress will also be dynamically studied after persistent heat stress on primary myocardial cells of chickens in vitro and the hearts of broilers in vivo under the heat stress.

为了探讨热应激鸡在应激状态下,尤其是应激早期,所呈现的高猝死现象与同期于心肌中Hsps蛋白表达出现显著降低的相关性,以及能否通过人工诱导Hsps过表达以预防心肌应激性损伤的发生，减少应激动物死亡率等问题，本研究拟在实验室可控环境和正常养殖环境下，分别对活体受试鸡和离体培养鸡心肌细胞采用药物（阿司匹林）预处理以诱导Hsps过表达。在此基础上进行持续热应激处理，动态观察活体受试鸡和离体培养鸡心肌细胞在受到热应激刺激后鸡心肌细胞中构成型和诱导型hsps mRNA转录水平、Hsps表达水平与心肌细胞应激性病理损伤及其程度的相关性，研究Hsps在活体受试鸡和离体培养鸡心肌细胞中的生理功能及在应激过程中的保护作用，探讨Hsps发挥保护作用的细胞信号转导通路，揭示热应激状态下Hsps表达水平的变化对心肌细胞抗应激损伤以及猝死发生的分子机理，拓展Hsps在畜禽应激损伤领域的深入研究。

为了探讨热应激鸡在应激状态下,尤其是应激早期,所呈现的高猝死现象与同期于心肌中Hsps蛋白表达出现显著降低的相关性,以及能否通过人工诱导Hsps过表达以预防心肌应激性损伤的发生，减少应激动物死亡率等问题，本研究拟在实验室可控环境和正常养殖环境下，分别对活体受试鸡和离体培养鸡心肌细胞采用药物（阿司匹林）预处理以诱导Hsps过表达。在此基础上进行持续热应激处理，动态观察活体受试鸡和离体培养鸡心肌细胞在受到热应激刺激后鸡心肌细胞中构成型和诱导型hsps mRNA转录水平、Hsps表达水平与心肌细胞应激性病理损伤及其程度的相关性，研究Hsps在活体受试鸡和离体培养鸡心肌细胞中的生理功能及在应激过程中的保护作用，探讨Hsps发挥保护作用的细胞信号转导通路，揭示热应激状态下Hsps表达水平的变化对心肌细胞抗应激损伤以及猝死发生的分子机理，拓展Hsps在畜禽应激损伤领域的深入研究。