单胺氧化酶基因与注意缺损多动障碍的连锁及其表达研究

Objective: to study the association between MAO gene and attention dificit.hyperactivity disorder (ADHD). Methods: eighty–four nuclear families with ADHD.were involved. Two X-linked microsatellites, (CA)n repeats in MAOA gene and (GT)n repeats in MAOB gene were determined with amplified fragment length polymorphism techniques. HHRR and TDT were used to analysis the association between the two.polymorphisms and ADHD. Competitive RT-PCR technique was used to detect the.expression of MAOB gene. Results: ①the association between MAOA gene and ADHD was observed(by TDT, χ 2=15.25,df7,P ＜ 0.05),but there was no association between MAOB(GT)n loci and ADHD（by TDT，χ2=11.18, df 7, P＞0.05）. ②there was no significant difference between the MAOB gene expression of ADHD(0.0183±0.0039 amol/ug cDNA) and that of healthy children（0.0181±0.0080 amol/ug cDNA）. Conclusion: maybe there.is no susceptible gene in Xp1 region of human X chromosome .

应用分子生物学技术研究注意缺损多动障碍（ADHD）与单胺氧化酶（MAO）基因关担辈捎镁赫鵕T-PCR技术测定ADHD儿童中MAOB型基因的表达水平，从而在分子水平上论DHD的基因定位及病因分析。预期将提出病因的新见解，为提高学龄儿童的精神健康提供杏τ眉壑档睦砺刍。倍訟DHD遗传咨询、临床治疗和预防将起极其重大意义。.........