大剂量照射对血管平滑肌细胞DNA修复基因表达谱的影响

Object：To observe the effect of γ-ray irradiation on rat vascular smooth muscle cells（VSMCs）and investigate the mechanism of inhibitory effect for γ-ray irradiation on VSMCs. Methods：Dose-survival curve of VSMCs was figured by colony formation. The effect of γ-ray irradiation on the proliferation of VSMCs was observed by 3H incorporation. Flow cytometry and "DNA Ladder " was used to detect the apopotogenic effect and cell cycle change of γray irradiation on VSMCs. "Western Blot" was used to investigate the expression of Tp53,cyclinD and PCNA after γray irradiation. .Results ：The values of D0,Dq,D37 and N for VSMCs were 1.95Gy, 1.76Gy, 3.71Gy and 2.47 respectively. The inhibitory effect of γ-ray irradiation on VSMCs proliferation was dose-dependent, being stronger along with increase of dose. VSMCs did not undergo apoptosis within 48 hours after γ-ray irradiation. VSMCs was arrestted in G1 phage afterγray irradiation. The expression of p53 was increased afterγ-ray irradiation, while the expression of cyclinD and PCNA was decreased.Conclusion：γ-ray irradiation could inhibit the proliferation of VSMCs , the main mechnism of which is the cell cycle arrest, killing effect and inhibition of mitosis of VSMCs after irradiation. p53、cyclinD and PCNA played an important role in this course. γ-ray irradiation could not induced VSMCs in vivo apoptosis .

血管内照射预防血管成形术后再狭窄是老年心血管阻塞性疾病研究领域的全新课题。探讨不同剂量照射条件下和照射前后原代血管平滑肌细胞，传代平滑肌细胞和动物整体水平的各类DNA修复基因表达图谱的变化规律，并观察其细胞学行为的改变对于在基因水平上揭示大剂空丈涠栽傧琳种频牧菩Ш透慕し涝傧琳闹瘟品椒ň哂兄卮蟮睦砺垡庖搴凸憷牧俅灿τ们熬啊