基于“阴平阳秘”理论从Pink1/Parkin通路探讨UU感染性不育症的“线粒体自噬失衡”机制及知柏地黄丸的干预作用

Infertility is one of the major diseases to threat men's health. Reproductive infection is one of the important factors. Infertility caused by iUreaplasma urealyticum (UU) infection results from kidney Yin deficiency and damp heat stasis, and the main performance is low sperm motility. It relates with the energy metabolism dysfunction caused by mitochondrial autophagy imbalance. Based on the theory of "Ying yang equilibrium", we put forward that "mitochondrial autophagy imbalances" is the microcosmic basis of kidney Yin deficiency. This study sets the mitochondrial autophagy as the breakthrough point, tries to demonstrate the association between decreasing sperm motility of UU infection infertility and mitochondrial energy metabolism disorder by mitochondrial autophagy. In this study, we set UU infection animal and cell model as the subject and apply the electronic microscope and Western blot techniques. We try to study whether Zhibai Dihuang pill improve the sperm motility by regulating mitochondrial energy metabolism through the mitochondrial autophagy and resistance to oxidative stress. This study will illustrate and enrich the connotation of "Yin yang equilibrium" from a new perspective. Furthermore, it will provide experimental proof to establish treatment strategy for UU infective infertility by “nourishing kidney yin, balancing yin and yang”.

不育是威胁男性健康的重大疾病之一，生殖系统感染是导致男性不育的重要因素，解脲脲原体（UU）感染性不育发病于肾阴亏虚、湿热瘀滞基础之上，精子活力低下是其主要表现，与“线粒体自噬失衡”所致生精细胞能量代谢障碍有关。基于“阴平阳秘”理论，我们提出UU感染性不育肾阴亏虚的微观基础是生精细胞“线粒体自噬失衡”。鉴于此，本研究以线粒体自噬为突破口，试阐述UU感染性不育精子活力下降与Pink1/Parkin介导线粒体自噬导致线粒体能量代谢障碍相关性。同时采用知柏地黄丸为施加因素，以UU感染动物及细胞模型为受试对象，运用电子显微镜、Western-blot等技术，研究知柏地黄丸提高UU感染大鼠精子活力是否通过调控生精细胞线粒体自噬，抗氧化应激，调控线粒体能量代谢，进而提高精子活力。本研究将从全新角度阐释和丰富“阴平阳秘”理论内涵，并为临床确立“滋肾阴、平阴阳”的UU感染性不育症中医药治疗策略提供实验依据。

解脲脲原体(UU)感染引起精子活力不足而导致的男性不育症是现代医学研究亟待解决的难题之一。UU感染性不育发病于肾阴亏虚、湿热瘀滞基础之上，精子活力低下是其主要表现，与“线粒体自噬失衡”所致生精细胞能量代谢障碍有关。. 基于“阴平阳秘”理论，我们提出UU感染性不育肾阴亏虚的微观基础是生精细胞“线粒体自噬失衡”。鉴于此，本研究以线粒体自噬为突破口，试阐述UU感染性不育精子活力下降与Pink1/Parkin介导线粒体自噬导致线粒体能量代谢障碍相关性。同时采用知柏地黄丸为施加因素，以UU感染动物及细胞模型为受试对象，运用电子显微镜、Western-blot等技术，研究知柏地黄丸提高UU感染大鼠精子活力是否通过调控生精细胞线粒体自噬，抗氧化应激，调控线粒体能量代谢，进而提高精子活力。本研究从全新角度阐释和丰富“阴平阳秘”理论内涵，并为临床确立“滋肾阴、平阴阳”的UU感染性不育症中医药治疗策略提供实验依据。