基于WGM光纤共振腔的中成药有效成分含量检测方法研究

Quality evaluation has been the focus and difficulty of the research and application of traditional Chinese medicine. Based on the research situation at home and abroad, according to the features of Chinese medicine like complex composition and great varieties, this project proposed a whispering gallery mode (WGM) cavity sensor to detect the content of effective component of Chinese patent medicine. The method of combining the theoretical analysis, numerical simulation and experimental verification is used to study the response of silica microsphere to the thin layer and particles adsorbed on the surface and the way to filter the dense and complex resonance spectrum, as well as the way to eliminate the temperature cross sensitivity of silica microsphere resonator. Taking fiber-based silica microsphere resonator and micro-fluidic tubular resonator as main sensing elements to design all-fiber sensors with high quality factor, high sensitivity, fast response and low cost. The sensor is used to detect the content of some kind of effective substance in Chinese patent medicine, and provide a new measurement for the quality evaluation standard of Chinese medicine.

中药品质评价一直是中药研究和应用的重点和难点，本项目基于目前国内外的研究现状，针对中成药具有的成分复杂、种类繁多的特点，提出基于WGM共振腔的中成药有效成分含量检测传感器，采用理论分析、数值模拟和实验验证相结合的方法研究二氧化硅共振微球对表面吸附薄层的响应、复杂且密集的二氧化硅微球WGM谐振光谱的滤波方法、消除二氧化硅微球共振腔温度交叉敏感的方法等，并将以光纤为原料制作的二氧化硅微球共振腔和便于实现微流操作的管状共振腔作为主要传感元件，设计能够产生高品质因子、高灵敏度、响应速度快、成本低廉的全光纤结构传感器，实现对安宫牛黄丸、西洋参等中成药中有效物质含量的检测，为中药品质评价标准提供新的测量方案。

本项目基于回音壁模式微腔生化传感技术，搭建了一套高Q值回音壁模式共振传感系统，实现了低检测限的牛血清白蛋白和中药厚朴中的有效成分Magnaldehyde B检测。本项目的研究内容包括：设计并制作细颈微球腔，与传统光纤端微球腔比推球率降低62%，保证其共振光谱的品质因子Q值在空气中达到107，在液体中达到106；设计并制作了一种非对称空心微球腔结构，通过施加轴向应变，实现了共振光谱可调，并研究了其应变传感特性；结合理论和实验，研究了WGM 共振腔表面吸附粒子的理论模型，研究了微球腔的折射率传感特性，并以牛血清白蛋白和中药厚朴中的有效成分Magnaldehyde B为待测物研究了生化传感特性。本项目的研究成果将为回音壁模式共振腔生化传感的研究提供实验依据和理论参考，极大丰富先进光纤传感技术的研究内容，促进智能化、微型化的生化检测方法应用发展。