水体中双氯芬酸与铜复合污染对鲫鱼的毒性效应及作用机制

Diclofenac (DCF), a non-steroidal anti-inflammatory drug, is widespread in aquatic environments and coexists with heavy metals (e.g., copper, Cu) to form combined pollution, posing an unpredictable threat to the aquatic ecosystem. It has been demonstrated that the complexation between DCF and Cu can influence the bioactivity of DCF in the pharmaceutical research. However, their complexation in waters and joint effects on aquatic organisms are still unknown. This project plans to study the complexation and its reaction mechanism between DCF and Cu using the method of UV and IR spectroscopy. To reveal the effects of complexation on the bioaccumulation and metabolic transformation of DCF, the distribution, accumulation and metabolism of both compounds are analyzed in crucian carp (Carassius auratus) using the methods of accelerated solvent extraction, solid phase extraction purification and mass spectrometry. The biomarkers of antioxidation, metabolism and prostaglandin synthesis at the cell and molecular level will also be investigated to clarify the joint effects and interactive mechanism of the combined pollution. The results will provide theoretical basis for evaluating the ecological effect of DCF, and are of great significance to the ecological risk assessment of complex pollution in aquatic environments.

非甾体抗炎药双氯芬酸（DCF）广泛存在于水环境中，并与铜（Cu）等重金属形成复合污染，对水生态系统健康构成威胁。DCF与Cu的络合作用在药理学上已证实会影响DCF的药物活性，但对于两者在水体中的络合作用及其对水生生物的联合效应尚不清楚。本项目拟采用紫外和红外光谱分析手段，研究DCF与Cu的络合效应及反应机理；运用加速溶剂萃取-固相萃取净化-串联质谱分析技术，解析DCF与Cu在典型水生生物鲫鱼体内的分布、累积和代谢物生成，揭示络合效应对DCF累积和代谢转化途径的影响；同时利用生物体抗氧化、代谢和前列腺素合成等系统功能在细胞和分子水平上的响应，阐明水体中DCF与Cu复合污染的毒性效应及作用机制。研究成果可为评价DCF在实际水环境中的生态效应提供理论依据，对水环境复合污染的生态风险评价具有重要意义。

非甾体抗炎药双氯芬酸（DCF）广泛存在于水环境中，并与铜（Cu）等重金属形成复合污染，但两者对水生生物的联合效应尚不清楚，给水生态系统健康带来难以预测的风险。本项目利用生物体抗氧化、代谢和解毒等系统功能在细胞和分子水平上的响应，研究了水体中DCF与Cu对鲫鱼的联合毒性效应。同时，运用超声萃取-固相萃取净化-串联质谱分析技术，解析了DCF与Cu在典型水生生物鲫鱼体内的分布和累积规律；此外，通过测定铜离子浓度和化学平衡软件进行模拟，揭示了Cu与DCF的络合作用。根据Cu和DCF的生物累积和络合作用规律，探讨了两种污染物联合毒性效应的作用机制。项目的研究结果可为评价DCF在实际水环境中的生态效应提供理论依据，对水环境复合污染的生态风险评价具有重要意义。