汉坦病毒感染细胞中信号通路的激活与炎症基因的表达

Hemorrhagic fever with renal syndrome (HFRS) is known to cause serious and often fatal human diseases, but the pathogenesis of it are still unclear. It is known that the damage of a number of tissues and cells of the body, especially the capillary endothelia cells, by hantavirus is primary and major mechanism of HFRS occurring and progressing. The research showed that abnormal immune modulation function played important role in HFRS occurring. To explore the effect of immune system to the pathogenesis of HFRS, the endothelial cells and human lymphocytes were infected by hantaan virus, and RT-PCR, immunofluorescence assay and Confocal Laser Microscope etc. were used to detected the expression of some cytokines and transcription factors in the infected cells. The results showed that the virus infection could induce different cytokines in human endothelial cells and human monocytes. In human endothelial cells, it could induce the expression of ICAM-1 and eNOS. However, it could induce the expression of IL-6 in human monocytes. Meanwhile, it is showed that hataan virus infected human monocytes could also result in the activation of IRF-3/IRF-7 , the expression of beta chemokine RANTES and the raise of Ca++ in cells. These results lays the foundation for further research on pathogenesis of HFRS.

本研究用汉坦病毒感染单核/巨噬细胞系和淋巴细胞系，用凝胶阻滞实验和虫荧光素酶报告靥寮觳飧腥鞠赴凶家蜃拥募せ睿舛っ富钚匀范ㄆ浼せ畹男藕磐罚冒攵縍T-PCR检测感染细胞中炎症基因的表达。本研究对进一步阐明HFRS的发病机理和免疫机制具有重睦砺酆褪导室庖濉