高压脉冲电场诱致皮肤结构性极化的研究

According to the epidemic investigation in 1998, it is shown that the incidence of disease of diabetes in the 26-64 years old crowd is 2.5%, there were 20.million diabetic patients in China. It is estimated that patients of diabetic will have been reached 60 million until 2010. The diabetes have becomes an important disease to threaten human health. However, so far the insulin has been the main specific medicine to treatment diabetes. Insulin is a kind of biological product with molecular.weight at 6,800 daltons. Various enzymes in stomach or intestine, or first pass of the liver degrade influence of insulin for breakage to oral delivery, and muscle injection.can suffer getting oneself restrict of operation technique and drug delivery time can 3 not compare with hour. Therefore, developing new route of drug delivery has be a very important challenge for biopharmacy industry and clinic.Transdermal drug delivery, other route to pass skin, overcome these.disadvantages of convention approaches, and offers a number of potential capacities.Nevertheless, very few drugs can be administered transdermally, due to the barrier properties of skin. Per-researches show that addition physical and chemical methods.can enhance drugs to permeate through skin, such as chemical enhancer, iontophoresis,sonophoresis, electroporation, photomechanical wave and magnetophoresis.Especially electroporation can increases macromolecular permeation to cross the.dermal. However the exact mechanism by which skin electroporation works is not.clear. The general goal of this project is to integrate electrician technique and biological approaches to design mini-device for drug delivery with novel binding properties. We would focus on the theory of mass transport through skin and the high voltage pulse electric field induces structural polarization of skin and characteristic.The project has the following assignments: (1) to research the theory of mass transport through skin of diffusion cell. It is assumed the diffusion cell is a open system, which have mass transport and energy transform, we should establish the.model of its network thermodynamics for which study quantificational relation.between pulse field and mass flux. (2) to research polarization of skin. We suggest that skin can be formed a new structure under electrical field because corneocyto and lipid domain are polarized cell and molecule. The structure increases the pore density.of the stratum corneum. (3) to develop an electroporation apparatus and diffusion cell.The specifications of the system are that pulse voltage: 50-400 V, pulse rate: 1, 2, 4, 8,.10, 20, 40 ppm, pulse number: 1-999 can count, energy capacity: super low, low,.middle, and high, anti-interference ability: isolation.In this project, we completed some experiments used the insulin as the model drug. The network thermodynamics model can predict the results showed that factors enhanced drug permeation through skin of pulse electrical field, and the reasons of the converse of skin resistance are analyzed by the theory of polarization of skin.

.本项目提出皮肤角质层脂质域局部极化理论。运用高压电与绝.缘技术、射频脉冲技术等电工新技术的理论与方法，从皮肤结构和.特性的动态变化来研究高压脉冲电场诱致皮肤电穿紫窒螅圆鞲哐孤龀宓绯《云し舻缃橹侍匦缘挠跋旃媛珊偷缁骰恚じ?药电穿孔技术的临床应用提供理论基础。研制新一代电穿孔仪和扩.散池，为本项目研究提供物质条?.