细胞器靶向的亚硫酸氢根荧光探针的设计合成及其在生物成像中的应用

Sulfur dioxide (SO2) is a main air pollutant which exists as sulfite and bisulfite anions in aqueous solution. Bisulfite are important chemical raw materials,which play important roles in human’s life and industries.However the overintake of bisulfite will cause serious deseases.Hence,it is very necessary to develop a satisfied detection method.Although a wide variety of techniques have been developed to detectbisulfite, fuorescence sensing has become the good standard due to its high sensitivity and simplicity. Thus, the construction of small-molecule fuorescent probes suitable for bisulfite detection in living systems has received great attention.Based on the literatures, we designed series of reaction based bisulfite fluorescent probes for the imaging of endogenously bisulfite in living cells. (1)Based on the intramolecular charge-transfer sensing mechanisms,mitochondria-targeted fluorescence probes for bisulfite were designed.(2) The mitochondria-targeted fluorescence probes for bisulfite in living cells were designed based on Fluorescence Resonance .Energy Transfersensing mechanisms.(3)An ICT-based fluorescence probe for visualizing bisulfite in lysosomes was developed.

SO2是一种主要的大气污染物，在水溶液中形成亚硫酸盐和亚硫酸氢盐。亚硫酸氢盐是重要的化工原料，在人类的生活、生产中发挥着重要的作用。但是过量的摄入会对人体造成严重的危害，因此，如何能够快速的检测他们是非常重要的。在各种识别和传感方法中，由于荧光法具有高灵敏度、设备简单、即时监测的优点，因而被化学家和生物学家广泛采用。本项目设计了基于亚硫酸氢根独特化学反应性的荧光探针，拟在亚硫酸氢根传感和生物细胞成像方面展开研究。本项目的创新点主要体现在：（1）基于ICT机制设计了线粒体靶向的亚硫酸氢根离子荧光探针，实现细胞器内亚硫酸氢根的定点检测。（2）基于FRET机制设计了线粒体靶向的亚硫酸氢根离子荧光探针，实现细胞器内亚硫酸氢根的定点检测。（3）开发有溶酶体靶向的荧光探针，实现细胞器内亚硫酸氢根的定点检测。

在过去二氧化硫一直是一种严重的环境和空气污染物。但是它的衍生物（主要是亚硫酸盐和亚硫酸氢盐）确被广泛应用于食品，饮料，和医药工艺，此外，二氧化硫还和呼吸系统疾病有关，所以迫切需要开发亚硫酸盐和亚硫酸氢盐的有效检测方法。基于ICT机制设计了线粒体靶向的亚硫酸根离子荧光探针，荧光探针展示了高度的选择性和专一型，能通过比率荧光和比色识别亚硫酸根。