寻址阵列编码多色近红外有机双光子荧光/核磁共振复合影像剂的制备及活体成像应用

Following the challenge of fluorescent agent with optical window in biological tissue for medical imaging diagnosis, the project mainly study the preparation of dual image agent multicolor NIR organic two-photon fluorescence/nuclear magnetic resonance (Am-NIRFP/MRI ) with addressable array coding and the applications in vivo imaging. Starting from the design of the molecular structure, a series of the multicolor near infrared organic two-photon fluorescence probes (m - NIRFP) with double biological window were prepared. The synthesis and design strategy for organic two-photon molecular was provided. Based on mechanism of target recognition, the simple preparation method was built for addressable multicolor nir organic two-photon fluorescence probes (Am - NIRFP) and the addressable specificity of detection for the object was achieved. Studying on the imaging performance and accuracy of the target positioning for the Am - NIRFP fluorescence, the impact mechanism of the molecular structure and fluorescence imaging performance in vivo was clarified. Which provided guidance for the development of Am - NIRFP with high quantum productive rate and high absorption cross section. Based on the regulation assembling of the nanomaterials with (super) paramagnetic, the resonance (NMR) reagent with T1 / T2 resonance relaxation nuclear magnetic which was array coding assembled with Am - NIRFP on the surface was obtained. And the dual image (Am - NIRFP agent/MRI) was prepared with the regulation performance. Then the detection method was built with multi-parameters, high-definition and targeted analysis in vivo. Collaborative relationships of dual imaging agent was discussed and the mechanism of the influence on the imaging effect for array coding structure was explicit. With the research of this project, the theoretical basis was provide for the construction and application of new multi-image agent. The testing platform was developed for in vivo imaging.

针对医学成像诊断中生物窗口荧光试剂目前面临的难题，该项目研究寻址阵列编码多色近红外有机双光子荧光/核磁共振复合影像剂的制备及活体成像应用。从分子结构设计出发，制备系列双生物窗口多色近红外有机双光子荧光探针；基于靶向识别机理，开发高量子产率和吸收截面的寻址多色近红外有机双光子荧光探针的简单制备方法，实现客体的可寻址特异性检测。研究其荧光成像性能及靶向定位准确性，阐明分子结构对活体成像效能的影响机理；基于（超）顺磁性纳米材料组装调控，获得T1/T2共振弛豫核磁共振试剂，在其表面阵列编码组装可调控的寻址多色近红外有机双光子荧光/核磁共振复合影像剂，构筑生物活体检测领域多参数、高清晰、靶向分析检测方法；探讨复合影像剂的协同关系，明确阵列编码结构对成像效果的影响机制，为发展生物活体一站式多参数检测方法提供策略，为新型复合影像剂的构建及应用提供理论依据，开发生物活体成像多手段同时检测平台。

该项目研究了靶向多色近红外有机双光子荧光/核磁共振复合影像剂的制备及活体成像应用。从分子结构设计出发，制备了系列近红外有机双光子荧光探针；基于靶向识别机理，开发了高量子产率和吸收截面的靶向多色近红外有机双光子荧光探针的简单制备方法，实现客体的靶向可寻址特异性检测。研究了其荧光成像性能及靶向定位准确性，阐明了分子结构对成像效能的影响机理；构筑了生物活体检测领域多参数、高清晰、靶向分析检测方法；为发展生物活体一站式多参数检测方法提供策略，为新型复合影像剂的构建及应用提供理论依据。