啶氧菌酯对斑马鱼多代毒性效应及分子机制研究

Picoxystrobin is the key fungicide against brown spot of Angelica sinenis in gansu province,and high toxicity for fish and frogs,with potential ecological risk.the preliminery study showed that the picoxystrobin had strong teratogenic effects on embryos of zebrafish, high lethal effect for adult fish and influence on the related enzyme activity.in this work,zebrafish were chosen as the research object,based on the effect on the zebrafish’s acute and subacute toxic index of picoxystrobin,the multi generation toxic effect of picoxystrobin with low dose and long touch time was studied in the angle of cell,molecule,omics level, furthermore,their toxic mechanism was explored in the molecular level, on the point of view of chemistry and biology, the residue-response mechanism of picoxystrobin were clarified.the study will provide a fundation for apply of picoxystrobin in high efficiency and low risk,and theoretical basis for a large number of safe application.

啶氧菌酯是防治甘肃省当归褐斑病的特效杀菌剂，但对当地水域中鱼、蛙类有较高毒性，具有潜在的生态风险。项目前期研究发现，啶氧菌酯对斑马鱼胚胎具有强烈的致畸效应，对成鱼剧毒，并对相关酶的活性产生影响。本研究拟以斑马鱼为研究对象，根据急性和亚急性毒性等毒性指标信息，从细胞、分子、组学水平研究低剂量长期暴露下啶氧菌酯对多代斑马鱼的毒性效应，进而在分子水平探讨啶氧菌酯对斑马鱼的致毒机理，从化学和生物角度揭示啶氧菌酯剂量-效应关系。本研究可为高效、低风险的使用啶氧菌酯奠定基础，也可为后期大量安全应用啶氧菌酯提供理论依据。

啶氧菌酯是甲氧基丙烯酸酯类杀菌剂，但是对水生生物高毒。项目研究发现，啶氧菌酯对斑马鱼胚胎、仔鱼、幼鱼、成鱼均为高毒，但其两种代谢产物对斑马鱼胚胎均为低毒。成鱼暴露啶氧菌酯后，对体重、体长、肠重等无明显影响，肠道微生物测序结果显示，对微生物多样性、丰富度、种类等无明显影响，群落结构显示，啶氧菌酯对雄鱼影响较大。胚胎暴露啶氧菌酯后，转录组分析结果表面，主要影响核糖体生成等。成鱼暴露50 μg/L啶氧菌酯30天，后对后代产生严重致畸效应，对胚胎的代谢组学显示，差异代谢物主要为肌酐类化合物，主要影响嘌呤代谢，差异基因为：rassf2b、foxq2、gpx7等，影响NOD受体等。研究结果显示，高浓度啶氧菌酯暴露，会对斑马鱼后代造成严重的影响。