大气压等离子体对健康皮肤的影响及作用机理研究

In recent years, plasma bio-medicine has been developing rapidly, and this science has been widely applied in the field of skin sterilization, wound-healing as well as the skin rejuvenation. However, in the process of plasma treatment, healthy skin around the treatment point will be unavoidably affected by the radiation from plasma, but in fact such an effect and the mechanism has rarely been reported so far. In this work, we designed an atmospheric plasma device with an air shield and adjustable electricity-discharging area, and the discharge characteristics of different plasma will be studied through the high-resolution spectroscope, ICCD high-speed camera, electrical probe and the infrared thermal imager. With regard to the living biological model skin tissues treated by different plasma, the changes in the cell morphology, structures, and the components of these tissues will be analyzed via biomedical methods using the HE stain, Masson stain, immunohistochemistry, fluorescent and luminescent probes, with the aim of investigating the extent to which plasma has infiltrated into the skin, the active particles damaging the skin, defining the safe parameters of the plasma treatment, and revealing the mechanism of atmospheric plasma on healthy skin. As a consequence, the studies into this program will not only provide the theoretical and experimental bases for the clinical application of plasma, but also the valuable reference to a safer clinical application.

近几年，等离子体生物医学发展迅速，在皮肤杀菌，伤口愈合，美容抗皱等方面具有广阔的应用前景。但在等离子体治疗过程中，病灶周围的健康皮肤难免也会受到辐射作用，而等离子体对于健康皮肤的影响及作用机制则鲜有报道。在本课题中，我们设计一种带有空气屏蔽罩且放电区域可调的等离子体发生装置；利用高精度光谱仪、ICCD高速相机、电学探头、红外热成像仪等仪器研究不同条件等离子体的放电特性；对等离子体处理过的活体生物模型皮肤组织，利用HE染色、Masson染色、免疫组化、荧光探针等生物医学方法对皮肤细胞的形态、结构、成分的变化进行分析，探究等离子体对皮肤的渗透深度，损伤皮肤的活性粒子，确定等离子体对皮肤作用的安全参数范围，揭示大气压等离子体对活体生物皮肤的作用机理。本课题的研究，为等离子体在生物医学的临床应用提供了理论及实验基础，为等离子体更安全的应用于临床提供了有价值的参考。

近几年，等离子体在生物医学领域的应用受到了越来越多的重视，本项目针对活体生物实验，设计了带有屏蔽罩的等离子体发生装置，利用高压探头、光谱仪、红外热成像仪等测量了该装置所产生的等离子体的放电特性、发射光谱、温度分布等特性。同时利用此装置对健康的活体SD大鼠皮肤进行了处理，对处理后的大鼠皮肤进行了HE染色、以及荧光探针检测，检测结果发现等离子体剂量控制不当将对健康皮肤造成一定的损伤，驱动电压、工作气体成分、工作距离等都会对等离子体成分产生影响，损伤皮肤的应激反应也会阻碍活性粒子向皮肤内部渗透。本项目的研究，对等离子体生物临床应用具有重要的参考意义。