电针治疗恒河猴麻黄碱成瘾的脑结构和功能的多模态磁共振成像研究

Drug addiction is one of the most serious social disasters all over the world. Bigotry psychological dependence, which results in the up to 95% relapse-rate, becomes a tough issue in medical field. Traditional low-frequency electro-acupuncture has a favorable therapeutic effect on physical dependence on drugs. However, the electro-acupuncture role on psychological dependence, especially on the relapse effect and mechanism induced by drug-related environmental cues are seldom researched. The study was designed to establish rhesuses models of ephedrine addiction by conditioning stimulation of rings and then to give multi-modle functional magnetic resonance imaging (fMRI) scanning of the ephedrine addiction experimental group after electro-acupuncture (EA) at different time. Another purpose of the study was to analyze the correlation between the multi-model fMRI manifestation and the behavioral and pathological changes of ephedrine addiction rhesuses, and to analyze the variation of related multi-model activated brain areas of ephedrine addiction rhesuses and changes of related activated brain areas after undergoing EA.Moreover the study aimed to explore the possible target site and functional connectivity approach of ephedrine addiction with acupoint EA and to provide scientific basis for the acuponit specificity and brain neuromechanism of electric acupuncture stimulation.

药物成瘾是最严重的全球性社会公害之一。顽固的心理依赖使得复吸率高达 95％以上，成为困扰医学界的难题。传统低频电针对药物的躯体依赖有较好的治疗作用，而 电针对于精神依赖，特别是药物相关环境线索诱发的复吸作用及机制研究还很少。本课题以铃声刺激建立条件反射的恒河猴麻黄碱成瘾模型，然后行电针治疗；对麻黄碱成瘾恒河猴不同时间段行多模态磁共振脑成像扫描，分析多模态磁共振脑成像表现与麻黄碱成瘾恒河猴行为改变及病理改变的相关性，分析麻黄碱成瘾恒河猴相关多模态磁共振脑区的变化规律，研究电针治疗麻黄碱成瘾相关脑区的变化，探寻电针组穴治疗麻黄碱成瘾的可能靶位及功能联接途径，为穴位特异性及电针刺激的多模态磁共振脑成像研究提供科学依据。

药物成瘾已是当今世界性医学难题，严重威胁人们的生活与健康。麻黄碱是当今世界新一代毒品，在世界很多地区蔓延，并且有明显增加的趋势。目前为止仍没有快速、有效、彻底的戒断方法来治疗药物成瘾。传统低频电针对药物的躯体依赖有较好的治疗作用，而电针对于精神依赖，特别是药物相关环境线索诱发的复吸作用及机制研究还很少。本课题以铃声刺激建立条件反射的恒河猴麻黄碱成瘾模型，然后行电针治疗；对麻黄碱成瘾恒河猴不同时间段行多模态磁共振脑成像扫描，分析多模态磁共振脑成像表现与麻黄碱成瘾恒河猴行为改变及病理改变的相关性，分析麻黄碱成瘾恒河猴相关多模态磁共振脑区的变化规律，研究电针治疗麻黄碱成瘾相关脑区的变化，探寻电针组穴治疗麻黄碱成瘾的可能靶位及功能联接途径，为穴位特异性及电针刺激的多模态磁共振脑成像研究提供科学依据。.麻黄碱成瘾恒河猴的行为症状、脑多模态磁共振脑成像及病理、分子生物学检测的结果分析，验证了成瘾恒河猴行为改变与脑功能变化及分子调控的相互关系，提示前额叶皮质、海马脑区是麻黄碱慢性成瘾的重要神经解剖结构，并参与了成瘾的神经功能调控；前额叶皮质、海马脑区中的CREB和CRF表达的变化是麻黄碱慢性成瘾的重要分子机制，其上调可能参与了麻黄碱慢性成瘾的生理病理和行为过程；前额叶皮质、海马可能是作为一个整合的神经回路（海马-前额叶回路）在麻黄碱慢性成瘾中发挥作用。本课题研究探讨麻黄碱慢性成瘾及其针灸治疗的相关功能联接途径及相关神经分子机制，将为临床上利用电针治疗新型药物成瘾提供科学的依据和指导。