中国南方喀斯特地区叶点霉属真菌分类与系统发育研究

The Phyllosticta is an important plant pathogen and endophytic fungi. Currently there are 180 known species in worldwide, and only 33 species were reported from China which indicates that there are many unknown Phyllosticta species remain to discover. The small conidia and few morphological characters bring many taxonomic difficult and several related genera species have been wrongly assigned to this genus and make the classification confusing. The Karst area of Southern China is a relatively unique geographical unit in China by having a variety of geological structure, altitude, topography, climate and abundant plant resources which are supposed to support a large number of fungal resources. With above advantages, we carry out the studies in southwestern karst area; the specimens of Phyllosticta will be collected and isolated. The taxonomic, phylogenetic and diversity studies will be taken based on the morphology and phylogeny studies. This study will present a good taxonomic and phylogenetic understanding of Phyllosticta in this area, and also provide a checklist of the Phyllosticta species which will be useful for the utilization of Phyllosticta, as well as the prevention and control of the plant pathogen.

叶点霉是一类重要的植物病原菌和内生菌。全世界已接受180种，但我国仅报道了33种。申请者对该属初步研究发现该菌孢子较小，分类特征少，技术要求高，导致大量近缘属真菌被归为该属，分类比较混乱；国内研究不多和样品收集区域不广，导致该属物种报道较少。中国南方喀斯特地区在世界上面积最大、发育最完全，该区域海拔差异大，垂直气候明显，具有独特的气候、地理及地质构造和丰富的植物资源，孕育了种类繁多的真菌资源。为此本课题组拟结合当地资源和自然条件，在该地区广泛收集叶点霉属病原菌标本，采用形态学结合分子系统发育分析的方法，对收集到的菌株进行系统发育、分类及多样性分析等研究；以期摸清该地区叶点霉属真菌种类、分布和系统发育关系，明确该地区叶点霉属真菌多样性。为充分发掘我国南方喀斯特地区叶点霉属真菌资源及该真菌的利用和病害的防治提供科学依据。