MSPD-DLLME联用分析血液中取代多溴联苯醚

Hydroxylated polybrominated diphenyl ether(OH-PBDEs) and methoxylated polybrominated diphenyl ethers (CH3O-PBDEs) have drawn particular attention in recent years as the persistent organic pollutants because they can result in thyroid dysfunction and nerve toxicity. It is a hot topic to establish the accurately detect method to detect accurately pollution level of the polybrominated diphenyl ether congeners in the human body using a small amount blood sample in environmental analysis and medical examination.On the basis of preliminary work, this project propose a new method that can achieve fast, green, high enrichment factor and recovery of ultra-trace analysis by adopting the combined technology of matrix solid phase dispersion (MSPD) and dispersive liquid liquid microextraction (DLLME) with solvent-free conversion to process blood samples. The project adopts matrix solid-phase dispersion (MSPD) method to process blood samples, firstly. The mixture of the appropriate supporter of MSPD and the blood sample is ground in a mortar to yield an apparent homogeneous blend, and the blend is transferred to a SPE cartridge. OH-PBDEs and CH3-PBDEs are separated by adopting appropriate solvent to elution the cartridge, and yield the eluent. Secondly, the eluent can be used directly in dispersive liquid liquid microextraction (DLLME) to obtain the micro upgrade samples which we need after DLLME treatment.Because the project combines the advantages of MSPD and DLLME which can eliminate many procedure and integrates the separation and enrichment, the analytical method greatly reduces the volume demanded of blood and the loss of analytical object and improves the analytical accuracy. The method has important academic and practical significance on the study of environmental behavior of polybrominated diphenyl ether congeners and other persistent pollutants.

羟基（OH-PBDEs）和甲氧基取代多溴联苯醚（CH3O-PBDEs）是近年人们特别关注的持久性有机污染物，它们会造成甲状腺功能紊乱和神经中毒。研究探索采取少量血样准确检测人体内取代多溴联苯醚的污染水平，是目前环境分析和医学检验的热门课题。本项目在前期工作的基础上，提出采用基质固相分散（MSPD）与分散液相微萃取（DLLME）无溶剂转换联用技术处理血样，从而建立一种快速、绿色、高富集倍数和回收率的超痕量分析新方法。该方法采用MSPD处理血样，选择合适的担体材料与血液样品研磨，得到均匀的混合物并将其作为填料装柱，选择合适的溶剂淋洗柱子；洗脱液直接用于DLLME，得到微升级检测样品。由于结合了MSPD和DLLME的优点，减少了多个环节，将分离与富集融为一体，从而大大减少血液用量和目标物的损失，提高分析准确度。该方法的建立无疑对研究取代多溴联苯醚等持久性污染物的环境行为具有重要的学术和实际意义。