长、短光周期敏感雄性不育水稻的育性调控差异蛋白研究

The two-line hybrid rice system has exhibit predominant advantages over the traditional three-line system. This technique system is based on the photoperiod-and thermo-sensitive genic male sterile rice that is a sterile line under long-day and high temperature environment and become a fertile line under short-day and lower temperature condition. Some short photoperiod sensitive male sterile rice germplasm was discovered in China, which was studied little in the past. The short-day sterile rice is a male sterile line under short-day and lower temperature but it becomes a fertile line under long-day and high temperature condition. The pair of reverse sterile rice is unique rice germplasm for the studies on expression and regulation of plant male sterile gene. The project aim to compare the character of tow kind of reverse sterile gene under different environments. Studies the differentiation expression in protein levels. The objective of this research is to characterize the gene expression exbasis of male sterile rice and wish to acquaint the ecology-genetic mechanism of reverse male sterile alteration reduced by photoperiod and temperature, and find out the respones protein relate to the male sterile gene and construe the basic fuuction of male sterile genes.

我国继长光周期敏感型雄性核不育水稻应用于两系法杂交水稻生产后，又发现了与之光周期敏感方向相反的短光周期敏感型雄性核不育水稻资源。申请者利用这两类独特的不育资源，育成了具有相同遗传背景、不育光照诱导条件相反的粳型近等不育系。项目拟对这两种不育系进行各自在不育和可育条件下的蛋白质表达分析，并比较这两种不育性之间的差异，对主要差异蛋白相关的基因进行不同光周期下的表达分析，试图从基因转录和蛋白质表达水平部分回答育性调控机制及两种不育类型的差异。研究对揭示植物雄性败育发生的遗传与生态调控代谢途径、不育基因功能有一定探索价值，对充分利用两种光敏不育基因资源有理论指导意义。

项目以长、短光敏雄性不育水稻为材料，在各自不育条件和可育条件下对幼穗、剑叶、叶鞘组织蛋白组、转录组差异进行了检测，比较分析了雄性不育特征蛋白和差异表达基因；可育差异蛋白和差异表达基因。利用蛋白组和转录组数据对获得的主要差异蛋白及相关基因进行了定量PCR验证、蛋白质Western Blot验证，对其中的酶蛋白进行相关酶活性测定。初步证实类黄酮代谢、茉莉酸代谢与信号通路水平差异参与调控光敏感雄性不育过程。建立起不育相关蛋白组和转录组数据库，为进一步研究光敏核不育水稻的育性转换机制提供了基础。发表标注项目资助研究论文8篇，其中sci收录论文5篇。获授权技术发明专利2项，副主编专著1部。培养博士后研究人员1名出站，博士生3人、硕士生3人毕业。