外源精胺对猪胎盘血管生成的作用及其分子机制研究

The insufficiency of placental function is one of the most important factors that cause embryo death and intrauterine growth retardation of baby pig. Placental function is closely associated with vascular density and diameter. Spermine is a kind of aliphatic amines that synthesized by cells and plays a very important role in endothelial cell proliferation and differentiation and lumen formation. In this project,an in vitro experiment with exogenous spermine to culture pig placenta microvascular endothelial cells and an in vivo experiment with exogenous spermine to feed Landrace × Yorkshire pregnant sows will be conducted to study the effect of exogenous spermine on placenta angiogenesis, polyamies metabolism and placenta vascular growth factors express with the methods of MTT, FCM, HPLC, immunohistochemical staining, Real-time PCR and western blotting. In addition, efforts will being focused on the elucidation of molecular action mechanism of exogenous spermine in placental angiogenesis by PI3K-AKT1-mTOR or MEK-ERK signaling parthway, which would contribute to provide academic and technical supports for the prevention of embryo death and intrauterine growth retardation.

导致母猪妊娠期胚胎死亡和仔猪宫内发育迟缓的因素很多，胎盘功能不足是其中最重要的因素之一。母猪的胎盘功能与胎盘血管密度和血管大小密切相关。精胺是由细胞合成的脂肪族胺类，具有促进内皮细胞增殖分化和加速管腔形成的作用。本项目通过外源精胺体外培养猪胎盘微血管内皮细胞实验和外源精胺饲喂长×大妊娠母猪体内试验，采用MTT法、流式细胞仪、高效液相色谱、免疫组化、实时荧光定量PCR和Western blotting等技术手段，利用体内和体外实验研究外源精胺对胎盘血管生成、妊娠母猪体内多胺的代谢规律和胎盘血管生长因子表达量的影响，并着重从PI3K—AKT1—mTOR或MEK—ERK信号通路探讨外源精胺在胎盘血管生成中的分子作用机制，为防治猪的胚胎死亡和宫内发育迟缓提供理论依据和技术支持。