应用PET脑功能成像技术研究针刺捻转补泻手法调控自发性高血压大鼠的中枢整合信号网络

Manipulation of twirling reinforcing-reducing of Acupuncture has been a controversial focus of Acupuncture and moxibustion cirle at home and abroad. This task group has found that the manipulation of twirling reinforcing-reducing has different biological effects and can regulating blood pressure by mechanisms of vascular endothelial growth factor, gene and protein expression in hypothalamus , vascular endothelial cell signal transduction pathways and neuronal discharge in the former National Natural Science Foundation of China. For further study on the effect mechanism of the manipulation of twirling reinforcing-reducing, this research select the spontaneously hypertensive rats as the research object, PET brain imaging technology ，RT-PCR technology and Western-blot technology as well be used to clarify the central integration mechanism of the manipulation of twirling reinforcing-reducing and construct the signal transduction networks of this manipulation on blood pressure regulation to clarify the effect mechanism of the manipulation of twirling reinforcing-reducing and provide a reliable theoretical support and technical method. for the prevention and treatment of hypertension.

针刺补泻手法长期以来都是国内外针灸界争议的焦点之一。本课题组在此前承担的国家自然基金课题研究中发现捻转补泻手法具有不同的生物学效应，并可以通过血管内皮因子、下丘脑基因和蛋白表达、血管内皮细胞信号转导通路和神经放电等机制调控血压。为进一步探讨捻转补泻手法的效应机制，本研究以自发性高血压大鼠为研究对象，利用PET脑功能成像技术，QT-PCR技术及Western-blot技术，阐明捻转补泻手法的中枢整合机制，构建捻转补泻手法调控血压的信号传导网络，并筛选出网络中的关键基因和蛋白，以期阐明捻转补泻手法的作用机制，同时为高血压的防治提供可靠的理论支持和技术方法。

为进一步阐明针刺捻转补泻手法调控血压的中枢效应机制，本课题以自发性高血压大鼠（SHR）模型为研究对象，以不同针刺捻转补泻手法作用于“太冲穴”为干预方式，运用PET脑功能成像技术，直观观察针刺捻转补泻手法对SHR各脑区的中枢激活效应，筛选出关键靶脑区并分析其与血压调控的生理病理联系。研究发现针刺捻转补泻手法可通过激活中枢靶脑区发挥其血压调控的作用，并且不同手法激活的脑区有差异；针刺捻转补泻手法可通过提高不同靶脑区的葡萄糖代谢水平发挥其降压的中枢效应，且干预周期不同，激活的靶脑区有差异；其潜在中枢机制可能与存在于延髓、下丘脑、海马、小脑、顶叶皮质等脑区中的神经递质及受体密切相关。本次课题进一步揭示了针刺捻转补泻手法调控血压的潜在中枢机制，同时为临床高血压的防治提供新的靶点和可靠的理论支撑。