循环MiR-210介导低氧对神经干细胞增殖的调控作用

Recent studies evidenced that the body fluids and cell culture supernatant contain plasma-based "circulating miRNA" which can enter specific cells to inhibit expression of target genes. Our previous study found that hypoxia can promote the secretion of miR-210 in vitro neural stem cell, and can increase circulating miR-210 content in the serum and cerebrospinal fluid. Combined with our previous findings, that exogenous miR-210 can promote neural stem cell proliferation, we present the scientific issues: "circulating miR-210 may be involved in the effect of hypoxia on the proliferation of neural stem cells". Our study will include the following three parts: ①the Effect of hypoxia on the secretion of miR-210 in vivo and in vitro;②the Effect of circulating miR-210 on the proliferation of neural stem cell in vivo and in vitro;③the regulation mechanism of hypoxia on the circulation miR-210. We may prove that circulating miR-210 is a new endogenous molecule which regulates the proliferation of neural stem cell, and clarify the molecular mechanisms in the same time. Our study may provide new methods for regulating the proliferation of neural stem cell as well as new strategies for the treatment of nervous system diseases.

近来研究发现体液或细胞培养上清液中存在着囊泡包裹的"循环miRNA" 。它们可以进入特定的细胞抑制靶基因的表达。我们前期研究发现低氧可以促进体外培养的神经干细胞分泌miR-210,也可以增加大鼠血清和脑脊液中循环miR-210的含量。结合我们以往发现外源性miR-210可以促进神经干细胞增殖的作用，提出"循环MiR-210可能介导了低氧对神经干细胞增殖调节" 的科学问题。通过研究：①低氧对在体脑脑积液和体外培养神经干细胞中miR-210分泌的影响；②循环miR-210对体外培养和体成年脑内神经干细胞增殖的调节作用;③低氧对循环miR-210调节的机制;将可能证明循环miR-210是一个新的调节神经干细胞增殖的内源性分子,同时阐明相关的分子机制。为调控体外和体内神经干细胞的增殖，以及探索神经系统疾病的治疗提供新策略。