大麦多节茎分枝形态建成的基因表达调控网络解析

Branching and tillering characteristics of plant are closely related to plant morphology and biomass. In contrast to millet and sorghum, typical barley cultivars has no shoot branching capability. However, after the fine mapping and positional cloning, we identified and characterized the candidate genes for multi-node and shoot branching mutant. For the purpose of understanding the development and the molecular mechanism of barley multi-node and shoot branching morphogenesis. The next generation sequencing approach will used for transcriptome analysis of whole life cycles of mutant and its wildtype. The gene regulation network analysis of which may take great agronomical importance for improving of plant architecture and molecular breeding.

植物的分枝和分蘖特性与植株的形态结构和生物量大小关系密切。在禾本科作物中，麦类的地上部茎节间并不像谷子和高粱那样，具有分枝能力。本研究在完成大麦多节茎分枝基因的精细定位，筛选出候选基因和研究候选基因的时空表达和遗传调控机制基础上，采用高通量测序技术，通过全生育期基因表达谱分析，进一步探讨禾本科作物茎分枝发育与形态建成的分子机理，为株型育种和分子改良提供理论依据。

植物的分枝和分蘖特性与植株的形态结构和生物量大小关系密切。本研究在完成大麦多节茎分枝基因的精细定位基础上，确定了HvNAT 基因为导致大麦多节茎分枝表型的mnd-7H 基因，并通过通过全生育期基因表达谱分析， 明确了由HvNAT 参与的昼夜节律信号代谢通路是大麦多节茎分枝（mnd-7H）表型形态建成的关键基因调控网络，为进一步研究禾本科作物茎分枝发育与形态建成的分子机理，为株型育种和分子改良提供理论依据。