Ryanodine受体RyR1的晶体结构研究
基本信息
结项摘要
Ryanodine受体（RyR）是由四个亚基组成的钙离子释放通道，在肌肉兴奋-收缩耦联等生物学过程中起重要作用。冷冻电镜/三维重构研究得到了大量结构信息，包括RyR四重对称结构，胞浆复合体1-10结构域，CaM、FKBP12的作用位点，差异区在胞浆复合体上的定位；根据细菌钾通道晶体结构KcsA构建的RyR孔道的结构模型。 最近研究得到RyR1分辨率为9.6 ? 的空间结构，建立了N端序列两个同源模型，测定了胞浆区二级结构成分，恶性高烧与中央轴空结病（MH/CCD）相关的突变氨基酸也在同源模型表面得以定位。即便如此，RyR跨膜区和孔道仍有许多待解之谜，有待用分辨率更高的X-射线晶体学进一步研究。本研究室RyR二维结晶的成功揭示了RyR分子排列的高度有序性；申请者据此成功培养了RyR1三维晶体。本项目将优化结晶条件，测定RyR1晶体结构，阐明其结构与功能的关系和钙离子通道病的分子机制。
项目摘要
项目成果
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数据更新时间：2023-05-31
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