卵巢颗粒细胞前蛋白转化酶对卵母细胞分化发育的调控作用及其机制

Granulosa cells is the main cells in human ovary,which regulate the differentiation and development of oocyte through secreting cytokines,hormone, growth factors, and so on. In the other hand, oocyte promote proliferation and differentiation of granulosa cells by paracrine. Along with the development of ART, it becomes the hot point that the effect of granulosa cells on the development of oocytes. Proprotein convertase catalyse a simple biochemical reaction-the proteolytic maturation of proprotein substrates in the secretory pathway.Proprotein convertase has an essential role of in embryogenesis, and catalyse the maturation of a strikingly deverse collection of proprotein substrates. These range from growth factors and receptorx to extracellular-matrix proteins and even other protease systems tant control disease. Recently we found that human lueteinized granulosa cells expressed the member of proprotein convertase: PACE4 and PC7/8 highly by real-time RT-PCR. We also found that rh-FSH down-regulate while rh-LH up-regulate the expression of PACE4 from granulosa cells in time- and dose-dependent manner.We need further study the effect of PACE4 and PC7/8 on the function of granulosa cells, the relationship between PACE4 and PC7/8 and development of oocytes. It will help us understand the important role of proprotein convertases in the development of oocytes.

卵泡颗粒细胞是卵巢的主要功能细胞，是卵泡内的主要体细胞成分，其与卵母细胞间存在包括缝隙连接在内的细胞间广泛的复杂连接机制，起着调节卵母细胞发育的重要作用。前蛋白转化酶（Protrotein convertases,PCs）主要功能是剪切无生物活性的蛋白或肽链的前体，使之变成有活性的功能分子。我们新近研究发现人原代黄素化颗粒细胞高表达前蛋白转化酶PACE4和PC7/8。我们还发现人重组卵泡生成素（FSH）以时间剂量依赖的方式下调颗粒细胞PACE4的表达而人重组黄体生成素（LH）以时间剂量依赖的方式上调颗粒细胞PACE4的表达。这些研究结果将启迪我们进一步研究黄素化颗粒细胞表达的PACE4和PC7/8对颗粒细胞功能的影响；研究黄素化颗粒细胞表达的PACE4和PC7/8对卵母细胞分化发育的影响。本研究有助于深入了人颗粒细胞与卵母细胞分化发育的关系，为卵母细胞分化发育的研究开拓新的思路。

卵泡颗粒细胞是卵巢的主要功能细胞，是卵泡内的主要体细胞成分，其与卵母细胞间存在包括缝隙连接在内的细胞间广泛的复杂连接机制，起着调节卵母细胞发育的重要作用。前蛋白转化酶（Protrotein convertases,PCs）主要功能是剪切无生物活性的蛋白或肽链的前体，使之变成有活性的功能分子。我们新近研究发现人原代黄素化颗粒细胞高表达前蛋白转化酶PCSK6。我们研究证实人重组LH上调颗粒细胞PCSK6 mRNA 的表达。通过RNAi干扰技术我们证实PCSK6通过activin-A及TGF-β途径促进颗粒细胞凋亡。我们的研究证实人原,颗粒细胞高表达前蛋白转化酶家族成员PCSK6,在LH调控下参与对颗粒细胞功能的调控，可能进一步参与卵母细胞分化发育。