生物醌复合纳米探针的光电生物传感研究

A series of quinone analogues with excellent biological performance are synthesized and stable inorganic nanomaterials aimed at analytical detection are prepared. Here, we construct a multifunctional nanobiosensor using quinone analouges as surface-capping ligands to functionalize nanoparticles. This program is to investigate the specific interactions of quinone and important biomolecules/protein by the biomimetics of biological process using quinone functionalized nanobiosenser.Our goal is to study the functionalization of quinone on the nanomaterial surface and construct the biosensing model to explore the target recognition by in-situ electrochemistry, plasma resonance scattering spectra and fluoresence spectra, thereby investigating the real time characterization of different life activities in cell. We believe our constructed model may hold particular promise as a powerful biosensor targeting in early stage disease diagnosis and progression monitoring based on nanobiosensing and biochemical detection technique.This will provide a new idea for cellular and molecular level reseaches in life sciences and the clinical diagnostics of disease.

本项目拟通过合成一系列具有特殊功能的生物醌类似物，开发针对分析检测稳定的功能纳米材料，构建靶向性分子识别、高灵敏传感的多功能纳米生物传感器，通过人工模拟醌的生化过程研究醌与重要生物活性分子、蛋白之间的作用。通过电化学、等离子共振散射光谱及荧光光谱等多种原位表征技术研究生物醌分子在纳米材料表面的固定化，构建生物传感识别模型应用于重要生物信号的靶向识别，从而实现对细胞内复杂生命活动的实时原位表征。在此基础上，将纳米生物传感技术与生化检测技术相结合，对疾病中出现的异常生物过程进行早期检测，为生命科学和临床诊断在细胞和分子水平上的研究提供新思路。

本项目通过合成和改性具有特殊功能的生物醌类似物( 如辅酶Q、多巴胺、 糖醌等)，开发针对分析检测稳定的无机纳米材料，利用自组装及静电吸附等相互作用方式构建生物醌功能化的高灵敏纳米生物传感器。利用原位光谱电化学联用技术，构建了纳米生物传感系统识别模型，实现了对与生物醌相关的生命体系中重要反应机制的原位追踪和血清样本中重要肿瘤标志物的超微检测。研究成果有望为仿生纳米材料制备、分析传感机理研究以及疾病检测等方面提供新方法和新思路。项目执行期间，项目负责人以第一或通讯作者已在Chem. Sci., Anal. Chem. (3篇), Theranostics, ACS Appl. Mater. Inter.等SCI杂志发表基金标注论文7篇，并获授权专利1项。