三磷酸腺苷参与内脏信息传递的机理

(1)With the Nystatin-perforated whole-cell patch-clamp recording technique, the.modulatory effects of adenosine on GABA-activated whole-cell currents were investigated.in neurons acutely dissociated from the superficial laminae (laminae I and II) of the rat.spinal dorsal horn..(2)With immunohistochemical staining technique, the distribution of P2X4 receptor was.observed in the rat nervous system..(3)With immunohistochemical staining technique, the distribution of metabotropic glutamate.receptors was observed in the sacral dorsal commissural nucleus of the rat spinal cord..(4)With immunohistochemical staining technique and immunoelectron microscopy, the.distribution of protein kinase Cγ subunit (PKCγ) in the rat nervous system was observed;.co-existence of PKCγ and calcium-binding proteins in neurons of the medullary dorsal horn.of the rat was observed; the ultrastructural localization of PKCγ and its synaptic connections.in the rat medullary dorsal horn..(5)With using fluroro-gold (FG) retrograde tracing combined with immunofluorescence.histochemical staining for PKCγ, PKCγ-containing neurons in the medullary and spinal.dorsal horns projecting to the midbrain periaqueductal gray, the nucleus raphe magnus and.gelatinosus thalamus nucleus in rats..(6)With immunofluorescence histochemical double-staining method, chemical neuroanatomy of.the superficial laminae of the mecullary dorsal horn were studied..国家自然科学基金资助项目结题报告.5.Major studying results.(1)The suppression of adenosine on IGABA is mediated by adenosine A1 receptor and through a.Ca2+-independent protein kinase C transduction pathway at the superficial laminae (laminae.I and II) of the rat spinal dorsal horn..(2)P2X4 receptor immunoreactive neuronal cell bodies were observed in the olfactory bulb,.anterior cingulated cortex, piriform cortex, medial septa nucleus, central amygdaloid.nucleus, hippocampal CA3 field, supramammilary nucleus, interpeduncular nucleus,.mesencephalic trigrminal nucleus, trigeminal motor nucleus, solitary tract nucleus, area.postrema, subfornical organ, Purkinje cell layer of the cerebellar cortex, laminateⅠandⅡof.the medullary and spinal dorsal horn, dorsal root ganglion and trigeminal ganglion..(3)The results of immunohistochemical staining showed that mGluRs-like immunoreactive.products were mainly located on the member of the neuronal cell bodies and fibers and in.the cytoplasm of the SDCN neurons and appeared as granular particles. A few large to.medium-sized mGluR1-like immunoreactive (mGluR1-LI) neuronal cell bodies were.observed in the SDCN, whereas mGluR1-LI fibers and terminals were seldom seen. Small.mGluR1α-LI neuronal cell bodies were sparsely encountered, while intensive network of.mGluR1α-LI fibers and terminals were detected in the SDCN. Some small to medium-sized.mGluR2/3-LI neuronal cell bodies, and scattered mGluR2/3-LI fibers and terminals were.observed in the SDCN. A few small mGluR5-LI neuronal cell bodies and quite dense.mGluR5-LI fibers and terminals were found in the SDCN. A few weakly stained.mGluR7-LI neuronal cell bodies, fibers and terminals were observed in the SDCN. Only a.few weakly stained mGluR8-LI terminal-like structure were sparsely encountered in the.SDCN. Dense mGluR7-LI neuronal cell bodies, fibers and terminals in the superficial.laminae of spinal dorsal horn were found, especially in the laminaⅠand inner part of lamina.Ⅱ. In the lateral spinal nucleus, some mGluR7-LI neuronal cell bodies were also observed..Only sparse mGluR8-LI structures were found in the spinal dorsal horn..(4)Intensely and densely stained PKCγ immunoreactive neurons were mainly observed in the.cerebral cortex, hippocampus, amygdaloid complex, cerebellar cortex, ventral and dorsal.cochlear nuclei, caudal spinal trigeminal nucleus and the superficial layers of the spinal.dorsal horn. Most of the PKCγ-, CB-, CR- and PV-immunoreactive neurons were observed.in lamina II; some were also encountered in lamina I and lamina III of the medullary dorsal.horn. Dense PK

本项目以接受盆腔内脏初级传入的大鼠骶髓后连合为靶区，在多种标本上应用膜片钳记录和细胞内钙离子测定等综合技术手段，研究三磷酸腺苷（ATP）及其代谢产物在内脏信息传递械淖饔煤突硪约八嵌怨劝彼帷⒏拾彼帷ABA等受体介导反应的调控作用及其机制、GABA等受体介导反应的调控作用及其机制。此项研究对阐明ATP在内脏信息传递中的作用机理有重要意义。