橡胶树乳管特异表达基因HMGR1启动子研究

Abstract.a method of genome walking was used to clone hev，ref, hmgr1 promoter, which then used in the transformations fusion with a uidA (GUS) gene to produce deletion constructs. In vitro expression studies of the deletion constructs showed that the -749- -292 sequence of hev promoter was sufficient to drive GUS reporter gene expression in latex, and sequence from -749- -168 could response to ABA inducement and promote the expression of GUS. In transient expression study, after particle bombardment, GUS was expressed only in veins of the leaf, and transformation of tobacco showed no GUS activity had been detected in veins of transgenic plantlets. This implies that hev promoter is a laticiferous specific promoter. .

通过对橡胶树乳管特异表达基因HMGR1启动子的分离、研究，构建橡胶树乳管特异表达的植镒靥澹雇庠椿蛟谌楣苤斜泶铩２⑼ü罱海栈裢庠吹鞍住４佣⒏叩戎参?橡胶)生物反应器－－这一极具潜力的生物加工体系打下基础。