天女木兰ABA受体蛋白Ms59134-PYR1在种子休眠解除中的功能及作用机制

Magnolia sieboldii K. Koch is a kind of rare and endangered wild plants resources tree species which focus on the protection of ornamental, aromatic and medicinal value in oneself. The morphological embryo hypoplasia, physiological after-ripping, and chemical inhibitors in seeds all make seeds to be dormant. The dormancy release is a complicated process. The molecular mechanism of Magnolia sieboldii seed dormancy release is still unclear. Our project group found that the ABA content of seed is closely related to dormancy release, the expression of Ms59134-PYR1 gene is significantly changed at both the transcriptional and translational levels during dormancy release, Ms59134-PYR1 and PP2C have protein-protein interaction sites and conservative sequences by the experimental research on the Physiological, Proteomics, Transcriptomics and Bioinformatics. Therefore, this project conducts in-depth study and discussion focusing on ABA signaling. This project tries to have a progress of Ms59134-PYR1 molecule biology, such as Gene Cloning, Expression System, Western Blot, Genetic Transformation and Yeast Two-Hybrid. Objective: To analysis functions of Ms59134-PYR1 gene, screen the interacting protein of Ms59134-PYR1, investigate the ABA signaling pathway. This project could provide scientific basis for the theory and practice of Magnolia sieboldii seed dormancy release.

天女木兰是国家重点保护的珍稀濒危植物。由于种胚发育不完全、种子中含有萌发抑制物等导致种子具有深休眠特性，极大地限制其开发应用。目前种子休眠解除的分子机制和调控网络仍不清楚。申请者所在课题组多年来通过天女木兰种子休眠解除过程中生理生化、蛋白质组学、转录组学的试验表明：天女木兰种子休眠解除与内源ABA变化相关，且ABA受体蛋白Ms59134-PYR1在转录和翻译水平显著变化，生物信息学分析进一步发现Ms59134-PYR1存在与PP2C相互作用的保守位点。因此，本项目将围绕ABA信号传导对种子休眠解除的调控这一科学问题开展研究，利用基因克隆、转基因、酵母双杂交等技术，研究Ms59134-PYR1蛋白在种子休眠解除中对ABA的响应，验证Ms59134-PYR1基因功能，筛选Ms59134-PYR1互作蛋白，初探天女木兰ABA信号传导途径。为深入探究木兰科植物种子休眠解除的分子机制提供科学的依据。