红细胞G6PD缺乏时溶血机理的研究

To explore the biochemical mechanism of G6PD deficiency．Metocds：A fluo-rescence polarization technique with l，6 -dipheny 1,1,3,5 -heratciene (DPH) as a fluorescent probe wasused to determine the fluidity of erythrocyte membrane lipid from G6PD deficient patients and normal controls. The polarization of fluorescence was measured between 00 and 900. Results: The fluorescence polarization parameter (ρ) WAS HIGH IN G6PD-deficient erythrocytes as compared with normal controls, indicating a decrease in erythrocyte membrane lipid fluidity. The determination of lipoperoxidation of erythrocyte membrane by fluorescence spectrometry also showed that the content of MDA was significantly higher in G6PD deficient erythrocytes than in normal controls. The percentage of unsaturated fatty acid C18:1 and C18:2 in total phosphalipids and phosphatidyl serine was decreased in G6PD deficient erythrocytes. Conclusion: The decrease of erythrocyte membrane lipid fluidity is related to thd lipid peroxidant, and the oxidative damage of erythrocyte membrane phosphalipids in G6PD deficient patients might be an important factor of hemolysis.

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