miR-137调控AQP2：阴道上皮细胞流体转运功能的新机制

Female Sexual Dysfunction (FSD) is a class of high incidence disease with potentially great harm, which has not been taken seriously. Vaginal lubrication disorder is one of the high incidence type of FSD. miR-137, is a novel miRNA significantly highly expressed in vaginal epithelium of vaginal lubrication disorder women with estrogen deficiency, compared to control based on our miRNA array data. Our previous study found that miR-137 is highly expressed in a variety of types vaginal lubrication disorder. Overexpressing of miR-137 in the VK2 / E6E7 significantly decreased the cell permeability. Bioinformatics and preliminary experimental indicated that AQP2 is the target gene of miR-137. The key function of AQP2 is water transport, which is closely related to vaginal lubrication. . Based on these results, we proposed that miR-137 may regulate fluid transport through its target gene AQP2 and contributed in the formation of vaginal lubrication disorder. To demonstrate this hypothesis, our study intends to evaluate the function of miR-137 in water transport in vaginal epithelium, based on cells, animals and clinical samples. The function and mechanism of miR-137 in formation of vaginal lubrication disorder has not been reported. This study will elucidate the regulatory mechanism, which may provide a theoretical basis for the targets of the diagnosis and treatment of vaginal lubrication disorder.

女性性功能障碍（FSD）是一类发病率高、潜在危害大，却一直未受重视的疾病。阴道润滑障碍是FSD的一种最高发类型。miR-137是我们前期筛选发现的差异高表达于阴道润滑障碍女性（雌激素缺乏）阴道上皮的miRNA。研究发现: miR-137在多种类型的阴道润滑障碍中均显著高表达；在人阴道上皮细胞系VK2/E6E7过表达miR-137显著降低细胞通透性；生物信息学和初步实验验证AQP2为其靶基因；AQP2具有促进水转运的功能，与阴道润滑密切相关。据此我们提出“miR-137通过AQP2调控阴道上皮细胞流体转运功能而参与阴道润滑障碍发生”的科学假说。本研究拟用细胞、动物和临床样本，以AQP2为机制线索，系统评价miR-137在阴道上皮细胞水转运中的功能和机制。miR-137在阴道润滑中尚无报道，本研究具有源头创新性，为理解阴道润滑障碍的病因机制提供新的线索，并可能提供新诊断和治疗靶标。

女性性功能障碍（FSD）是一种具有严重潜在危害的常见疾病，但尚未得到足够的重视。FSD的发病机制研究成为FSD诊断和治疗的迫切需要。本研究旨在探讨miR-137在女性性功能障碍中的作用。我们收集15例有润滑性疾病的女性阴道上皮组织和15例功能正常的女性阴道上皮组织。通过微阵列分析和实时定量PCR（qPCR）检测润滑病症和正常功能女性中miR-137的表达水平。通过慢病毒感染在阴道上皮细胞VK2/E6E7中过表达miR-137。使用钙黄绿素淬灭法测量细胞水渗透性。通过流式细胞术分析细胞凋亡。通过生物信息学分析预测miR-137的潜在靶标，然后通过荧光素酶报告基因验证进行验证。本研究发现miR-137在具有润滑障碍的女性的阴道上皮组织中高度表达。此外，研究表明，miR-137的过表达导致细胞渗透性降低。通过目的基因预测和实验验证相结合，我们确定AQP2是影响阴道上皮细胞水渗透性的miR-137的直接机制靶标，本研究可以为阴道润滑障碍及其相关疾病提供潜在治疗靶标。