miR-342-3p通过Hedgehog信号通路促成骨分化的机制研究

During craniofacial development, the Hedgehog (HH) signaling pathway is essential for mesodermal tissue patterning and differentiation. Hh signaling acts at multiple critical steps in controlling osteoblast proliferation and differentiation during bone development. In the previous study, we found one specific microRNA, miR-342-3p, was upregulated during osteogenic differentiation, which indicated that they may be involved in regulation of osteogenisis. As a result of overexpression of miR342-3p in hUCMSCs, SUFU, suppressor of GLI1, protein level was drastically decreased while osteogenic markers ALP and osteocalcin were upregulated during osteogenic differentiation. In this study, we investigate the mechanisms of miR342-3p modulates the Hh signaling pathway by regulating SUFU/Gli expression during hUCMSCs differentiation. We propose miR-342-3p to target molecules for promoting bone regeneration and wound healing in bone tissue engineering and clinic implantology.

Hedgehog（Hh）信号通路不仅是胚胎发育时期骨骼形成及发育的重要调控因子，还能调控出生后的骨组织，维持内环境的稳定性。我们在对Hh信号通路促成骨效应的研究中发现miR-342-3p在间充质干细胞中过表达能促进细胞成骨分化，同时Hh信号通路的负调控因子Sufu蛋白水平显著下降。这些研究结果提示miR-342-3p可能作为关键因子通过Sufu相关的信号轴调控Hh信号通路，促进干细胞成骨分化。在本项目中，我们致力于探讨miR-342-3p是否通过Sufu/Gli信号轴，在Hh信号通路的成骨效应中发挥调控作用。本研究的结果将有助于进一步探讨Hh信号通路在骨改建中的作用机制，并筛选出新的调控因子作为靶分子对种植体植入后的骨改建过程进行精准操控，为种植义齿骨组织工程提供新的治疗契机。

Hedgehog(Hh)信号通路不仅是胚胎发育时期骨骼形成及发育的重要调控因子，还能双向调控 出生后的骨组织，维持内环境的稳定性。我们在对Hh信号通路促成骨效应的研究中发现miR-34 2-3p在脐带间充质干细胞(hUCMSCs)中过表达能促进细胞成骨分化，同时Hh信号通路的负调 控因子Sufu蛋白水平显著下降。这些研究结果提示miR-342-3p可能作为关键因子通过Sufu相关 的信号轴调控Hh信号通路，促进干细胞成骨分化。在本项目中，我们致力于探讨miR-342-3p在 hUCMSCs中是如何通过Sufu在Hh信号通路的骨改建中发挥调控作用。本研究的结果将有助于进 一步探讨Hh信号通路在骨改建中的作用机制，并筛选出新的调控因子作为靶分子对种植体植入 后的骨改建过程进行精准操控，为种植义齿骨组织工程提供新的治疗契机。