草莓果实发育及成熟调控中FERL与ABA与信号转导 的交叉对话及整合机理研究

It has been well demonstrated that plant hormone, ABA, plays an important role in the regulation of strawberry fruit development and ripening. Our recent studies suggest that, besides ABA, FaFERL (an FER-like RLK) also plays important role in the regulation of strawberry fruit development and ripening. The basis for FaFERL to control strawberry fruit development and ripening is its cross-talk with ABA signaling. In the present research subject, we would further explore the mechanisms for the cross-talk and integration between the FaFERL and ABA signaling pathways. We would first characterize the ‘cross response’, ‘integration response’ and ‘independent response’ respectively mediated by FaFERL and ABA signaling, meanwhile, we would identify the specific members in the FaFERL signaling module. Moreover, we would explore the protein-protein interaction of the members between FaFERL and ABA signaling module, and particularly, we would explore the potential complex formation among FaFERL, FaPYL1/6 and FaABI1, explore the FaFERL-catalyzed phosphorylation and the function of the phosphorylation sites, so that deeply understand the mechanisms for cross-talk between FaFERL and ABA signaling. This research subject is of great significance for a deep understanding of the molecular basis of the strawberry fruit development and ripening and the molecular engineering of strawberry plants.

业已证明，ABA是操纵草莓果实成熟的核心信号。我们最近的研究表明，除ABA外，FaFERL同样在草莓果实成熟调控中起着重要作用。FaFERL操纵草莓果实成熟的机制在于和ABA信号通路发生交叉对话。本研究旨在进一步探讨FaFERL与ABA信号交叉对话和信号整合的机理。研究拟将首先解析FaFERL和ABA信号路径的‘交叉应答’、‘整合应答’和‘独立应答’特征，同时解析FaFERL分子识别信号模块的成员构成。在此基础上，深入分析FaFERL和ABA分子识别信号模块成员之间的交叉互作关系，以及FaFERL、FaPYL1/6和FaABI1之间可能存在的复合互作关系，揭示FaFERL对FaPYL1/6和FaABI1的磷酸化修饰及其磷酸化位点和功能，以深刻了解FaFERL和ABA信号交叉对话的机理。该研究对明晰草莓果实发育调控的分子机理，以期通过基因工程实现品种创新具有重要意义。

本研究旨在揭示FER和ABA信号转导在草莓果实发育合成熟中的作用和机理。FER从属于CrRLK家族，包括FERONIA和THESUES 亚家族，在植物发育中起着重要作用。脱落酸（abscisic acid, ABA）是操纵草莓果实发育和成熟的核心信号，其信号起源机制的揭示是主要研究内容。研究揭示了FER可以通过ABI2路径影响ABA信号转导，同时FER近源家族THE在ABA信号起源中起着重要作用。重要的是，研究揭示了ABA信号起源是一个自我催化放大过程，这些研究发现对进一步揭示草莓果实成熟和品质形成的关键基因，从而在分子育种中得以实际应用具有重要意义。研究取得丰硕成果，圆满完成既定任务，其体现在，以第一标注在高水平刊物Horticultural Research (IF7.291) 和Frontiers in Plant Science (6.627)各一篇；以第2标注在Horticultural Research (IF7.291)和International Journal of Molecular Science (6.208)发表论文3篇；另外在New Phytologist （IF 10.151）和Plant Physiology and Biochemistry （IF5.437）发表论文各一篇（第5和第3标注）。培养博士研究生4名，硕士研究生7名。