电针刺激通过调控杏仁核neuropsin通路改善大鼠胃高敏感及焦虑障碍

Gastric hypersensitivity was the main mechanism of functional dyspepsia (FD). But the effect of anxiety in the development of FD was also important. Electroacupuncture(EA) was an effective therapy in improving visceral hypersensitivity and anxiety, and the central mechanism might relate to changing the activity of vagus nerve, brain stem and amygdale. Amygdala was the main central area in controlling visceral sense and emotion. Recent research found that neuropsin in amygdala was the key factor in anxiety formation. Whether the gastric hypersensitivity and anxiety related to amygdala neuropsin pathway was still unknown. In this study, we will investigate the expression of the factors in amygdala neuropsin pathway in gastric hypersensitivity rats, and observe the controlling of it by exogenous inhibitor or EA, in order to clarify the central mechanism of gastric hypersensitivity and anxiety formation, and the possible mechanism of EA.

胃高敏感是功能性消化不良（FD）患者症状产生的主要机制，但焦虑等情绪障碍在FD发病中的作用仍不可忽视。FD时胃高敏感和焦虑障碍是相互作用的。电针刺激已被证实可改善内脏高敏感及焦虑样行为，其中枢作用可能与改变迷走神经、脑干及杏仁核活性有关，但确切机制不详。杏仁核是中枢调节内脏感觉功能和焦虑情绪的重要高级结构。近期研究发现，杏仁核内neuropsin是焦虑形成的关键因子。胃敏感性增高及焦虑障碍是否也与杏仁核neuropsin通路相关尚待研究。本课题拟通过研究胃高敏感时杏仁核neuropsin通路相关信号分子的表达及对其的调控，证实该通路在胃高敏感及焦虑形成中的作用，并通过研究电针刺激对该通路的影响，进一步阐明电针刺激的中枢作用机制。

胃感觉高敏感和焦虑情绪是功能性消化不良患者的主要症状。作为控制情绪、行为的中枢杏仁核区域调控内脏感觉的机制尚不清楚，并且，能有效改善消化不良症状的电针刺激发挥作用的机制亦不明确。本课题通过构建胃高敏感大鼠模型，研究胃高敏感时外周及杏仁核neuropsin通路的表达及功能，证实杏仁核neuropsin参与胃高敏感及焦虑样情绪形成，电针刺激通过抑制杏仁核neuropsin表达及功能，发挥降低胃高敏感和抗焦虑作用。