生物玻璃涂层对人工韧带生物相容性和腱骨愈合的影响及机制研究

As one of the hot spots in sports medicine, the selection of grafts adopted in the anterior cruciate ligament(ACL) reconstruction is still in consistent discussion.Up till now, great attention has been paid to the polyethylene Terephthalate(PET)-knitted artificial ligaments in domestic and overseas.Currently,the domestic artificial ligaments market was engrossed by the LARS products,while the defects of such prostheses was obvious,such as the poor biological healing in tendon-bone interface and the uncertainty of long-term prognosis.Our previous researches proved that the use of hydroxyapatite coating technique of PET artificial ligament could promote the tendon-bone healing in its bone-tunnel segment.In addition, the bioactive glass is a novel material featured with many beneficial effects such as:osteostimulative, osteoconductive, vascularization and anti-inflammation.Thus recently,the bioglass has been introduced in orthopedic application.This study is supposed to compare two different coating methods in the application of bioglass on the artificial ligament—the plasma spray technique and the solution deposition technique in both vitro and vivo.The characteristic of the materials and the cellular compatibility tests will conducted in vitro; then the ACL reconstruction model will be created to test the effect the method in vivo. To further compare outcomes such as biocompatibility and tendon-bone healing effect of the two methods, the biomechanical tests, the histological test and the Raman spectrum are sequently adopted to verify a better coating method.To further explore the mechanism of the signal pathways, the MAPK, the c-Jun signal pathways, and some osteogenesis and angiogenesis factors will be tested.Our research will provide foundation for the final clinical practice of this modified artificial ligament,both theoretically and experimentally.

膝关节前交叉韧带损伤重建手术的移植物选择是运动医学研究热点之一。有关聚对苯二甲酸乙二醇酯（PET）人工韧带的研究在国内外受到高度关注。国内人工韧带市场被进口LARS 产品垄断，但其存在材料与骨愈合不佳、远期效果不理想等缺陷。我们的前期研究表明应用材料表面羟基磷灰石涂层技术可有效促进其在体内与骨的愈合，但其效用仍较为单一。近年来生物玻璃因其具有骨传导性、骨诱导性、可促进血管生成、抗炎等诸多优点在骨科生物材料中得到广泛应用。本项目旨在应用新型易于产业化发展的等离子喷涂技术对PET材料进行表面生物玻璃涂层，并通过材料表征、体外细胞相容性及成骨性能检测及体内动物实验包括病理、生物力学及Raman光谱检测并与传统涂层方法进行对比研究，验证其体内外优势。同时对涂层后MAPK及c-Jun信号通路相关分子及体内成骨成血管因子进行检测，探索其内在机制，为研制新型符合临床要求的人工韧带提供理论和实验依据。

膝关节前交叉韧带损伤重建手术的移植物选择是运动医学研究热点之一。有关聚对苯二甲酸乙二醇酯（PET）人工韧带的研究在国内外受到高度关注。国内人工韧带市场被进口LARS 产品垄断，但其存在材料与骨愈合不佳、远期效果不理想等缺陷。我们的前期研究表明应用材料表面羟基磷灰石涂层技术可有效促进其在体内与骨的愈合，但其效用仍较为单一。近年来生物玻璃因其具有骨传导性、骨诱导性、可促进血管生成、抗炎等诸多优点在骨科生物材料中得到广泛应用。本项目采用低温等离子喷涂技术涂层羟基磷灰石-PET人工韧带，并在此基础上进一步复合辛伐他汀-壳聚糖生物活性涂层，通过材料理化性质表征、细胞实验及多种动物模型证实等离子喷涂处理后的PET韧带亲水性、生物活性显著增加，具有诱导成骨分化能力，并能有效增强骨整合进程，为研制新型符合临床要求的人工韧带提供了理论和实验依据。