基于单层WS2纳米片的新型核酸传感技术及其microRNA检测应用研究

MicroRNAs play vital roles in physiologic and pathologic processes and are significant biomarkers for tumor early diagnostics. Thus there has been aroused great attention for the development of sensing technology for the rapid, sensitive and selective detection of microRNA. In this project, via combining the technologies of nanomaterial preparation, the advantages of biosensor technology, systematic studies will be performed on the development of novel WS2 nanosheet-based biosensors on the basis of different affinity between various conformation nucleic acids and WS2 nanosheet, and the stability of biosensor in complex physiological circumstances. On this basis, a series of novel assay platform will be developed for sensitive, selective, and low-cost microRNA biosensing via the combining the technologies of nucleic acid amplification and WS2 nanosheet. This project will pioneer a new approach in both theory and experiment to apply WS2 nanosheets in biosensors and extend the research scopes of two-dimensional layered nanomaterials.

MicroRNA对人体生理和病理等过程具有重要调节作用，同时也是癌症早期诊断的生物标记物，因此发展快速、高灵敏、高选择性的microRNA检测技术近年来已经成为研究热点。本项目拟结合纳米材料制备和生物传感技术，系统性的开展对不同构象DNA具有吸附选择性的WS2纳米片生物传感器及复杂生理环境下生物传感器稳定性的研究，并在此基础上，进一步结合核酸放大技术，发展灵敏度高、选择性好、成本低的新型microRNA定量检测分析技术。本项目在理论和实验上开辟了WS2 纳米片在生物传感器应用的新方向，拓展了二维片状纳米材料的应用范围。

肿瘤标志物的检测与分析对癌症早期诊断意义重大，发展高灵敏、高选择性及快速检测技术是肿瘤检测领域的一项挑战性任务。本项目研究了系列纳米材料的制备、功能化及相关性能；探索了系列核酸介导的分子识别信号转换与放大技术；通过筛选优化所研究的纳米材料及核酸信号转换放大技术，以与肿瘤密切相关的生物分子如透明酸质酶、DNA、谷胱甘肽、抗坏血酸、碱性磷酸酶等为研究对象，建立了多种灵敏度高、选择性好的荧光检测新方法。相关研究成果已经形成ACS Sustainable Chemistry & Engineering，Sensors and Actuators B-Chemical等SCI收录论文18篇，均标注本项目资助，其中IF>4论文9篇，包括ACS Sustainable Chemistry & Engineering，Sensors and Actuators B-Chemical, Microchimica Acta, Talanta等生物传感及相关领域重要国际学术刊物的论文。同时，项目组培养了8名硕士生，基本完成了既定目标和任务。