亚麻抗白粉病基因Pm9801的克隆与功能解析

Flax (Linum usitatissimum L.) is one of the most important economic crops. Flax production in China takes an important role all over the world. Powdery mildew (Oidium lini Skoric) is a common disease of flax in most flax-growing areas of the world. The disease brings huge danger for the farmers and flax manufacturers,and it has become a most severe problem for flax planting. Based on thevariety of linum resource and the material 9801 which possessing strong resistance to Oidium lini, we have confirmed that the resistance is controled by one major gene,here temporarily designated as Pm9801. According to the study of SSR markers, finely mapping and sequencing, we identified a DNA segments associated with resistance to PM. Through analysis of gene expression, transcriptome sequencing, subcell localization and bioinformatics as well, we tend to reveal the powdery mildew Resistance gene such as Pm9801 functional pathway and molecular mechanism of powdery mildew resistance. In parallel, a molecular marker assisted selection system will be expected to establish, in order to efficiently apply the resistance gene to improve the major flax variety Diane,an elite France-introduced variety with many merits but for non-resistance of powdery mildew.

亚麻是我国的最重要经济作物之一。近年来，亚麻白粉病在我国日益频发，给亚麻生产带来重大的损失。可见，提高亚麻白粉抗性是当务之急。本课题组前期利用抗病品种9801与感病品种Diane杂交，鉴定了一个亚麻白粉病抗病基因Pm9801，运用SSR标记、转基因和测序技术，目前我们已经获得一个包含Pm9801的DNA片段。在此基础上，本研究拟在基因预测候选基础上，利用分子标记技术继续克隆抗白粉病基因，建立饱和分子标记。构建系列转化载体，转化感病品种Diane，最终克隆包括Pm9801在内的抗白粉病基因。并进一步通过基因表达分析、转录组测序、酵母双杂交、亚细胞定位以及生物信息学等技术，综合分析抗白粉病基因的分子代谢模式，阐明Pm9801抗白粉病的分子生物学机理。

亚麻是我国的最重要经济作物之一，其生产在世界上占据重要地位。近年来，亚麻白粉病在我国日益频发，给亚麻生产带来重大的损失，已成为急需解决的重要问题。培育抗病品种依然是病害防治的最佳策略。本课题组前期利用抗病品种9801与感病品种Diane杂交，鉴定了一个亚麻白粉病抗病基因Pm9801，运用SSR标记、转基因和测序技术，目前我们已经获得一个包含Pm9801的DNA片段。在此基础上，本研究拟在基因预测候选基础上，利用分子标记技术继续克隆抗白粉病基因，建立饱和分子标记。构建系列转化载体，转化感病品种Diane，最终克隆包括Pm9801在内的抗白粉病基因。并进一步通过基因表达分析、转录组测序、酵母双杂交、亚细胞定位以及生物信息学等技术，综合分析抗白粉病基因的分子代谢模式，阐明Pm9801抗白粉病的分子生物学机理。本研究不仅为利用Pm9801提供了精确的标记，同时也为抗病基因工程提供了遗传材料。