雪旺氏细胞联合成纤维细胞移植在皮瓣神经再生中的作用

Repairing the tissue defects often need a flap reconstruction, but the reconstruction of the sensory function of the denervated flap failed to be solved effectively. Flap restore its sensory function by promoting nerve regeneration is of great significance. The peripheral nerve has a strong regenerative ability after damaged and the regeneration mainly depends on the Schwann cells. Recent research indicates that after injury , the Schwann cells migrate from the nerve stumps to the " nerve bridge " and format a discrete cellular zone followed by the neonatal nerve axon into the " nerve bridge ". Fibroblasts regulating the Schwann cells form a cluster through the Ephrin/Eph Sox2 - Ncadherin signaling pathways . Thus mediates the growth of nerve fiber, promotes the orderly regeneration of nerve fibers . This project intends to establish a rat abdominal wall innervation of perforators flap, through schwann cells and fibroblasts co-culture in vitro.Then transplant to the receptive area. Observation flap nerve regeneration. This is a new method to promote skin flap nerve regenerationand provide some protophase basic research for clinical flap sensory function reconstruction.

组织缺损常常需要皮瓣进行修复重建，但是失神经支配皮瓣的感觉功能重建却未能有效解决。通过促进皮瓣神经再生恢复其感觉功能具有重要意义。外周神经受损后具有很强的再生能力，其再生主要是通过雪旺氏细胞完成。新近研究表明神经损伤后雪旺氏细胞从神经残端向"神经桥"迁移并形成不连续的细胞带，新生的神经轴突紧随雪旺氏细胞的迁移路径进入"神经桥"，在这过程中成纤维细胞通过Ephrin/Eph-Sox2-Ncadherin信号通路调控雪旺氏细胞形成簇群，从而介导了神经纤维的生长，促进了神经纤维有序再生。本项目拟建立大鼠腹壁失神经支配的穿支皮瓣，通过雪旺氏细胞和成纤维细胞体外共培养及皮瓣移植受区共移植，观察皮瓣神经再生情况。该方法是促进皮瓣神经再生的一新方法，研究结果对临床上皮瓣感觉功能重建提供一些前期基础研究。

组织缺损常常需要皮瓣进行修复重建，但是失神经支配皮瓣的感觉功能重建却未能有效解决。通过促进皮瓣神经再生恢复其感觉功能具有重要意义。外周神经受损后具有很强的再生能力，其再生主要是通过雪旺氏细胞完成，在这过程中成纤维细胞通过Ephrin/Eph-Sox2-Ncadherin信号通路调控雪旺氏细胞（Schwann cells, SCs）, SCs形成簇群，从而介导了神经纤维的生长，促进了神经纤维有序再生。本项目建立了大鼠腹壁失神经支配的穿支皮瓣，通过SCs和成纤维细胞体外共培养及皮瓣移植受区共移植，检测到Eph受体、转录因子Sox2和N-cadherin蛋白的表达水平升高，证明了成纤维细胞与SCs共移植可通过Ephrin/Eph-Sox2-N-cadherin信号通路调控SCs迁移并聚集成簇群，从而促进皮瓣神经再生。同时将人羊膜间充质干细胞（human amniotic membrane mesenchymal stem cells，hAMSCs）和其诱导得到的雪旺氏细胞样细胞（induce Schwann cells，iSCs）分别移植到失神经支配的皮瓣模型后，观察神经的再生，证明了hAMSCs和iSCs均可以促进神经的再生，其中iSCs通过高表达BDNF and NGF 对促进神经再生数量增多和神经纤维直径增粗有更好的作用。