疏肝利胆法对肝脏β－UGT和胆固醇7α羟化酶基因的调控

Objective: To probe the impact of the TCM therapy of dispersing stagnated liver qi and promoting bile secretion [DSPB] on the expression of gallstone-related genes Bilirubin-Uridine Glucnronosy Transferase (B-UGT) and cholesterol 7a-hydroxylase. Material and Method: An animal model of Pigment gallstone was reproduced using low-protein Lithogenic diet as we previously reported. Experimental guinea-pigs were randomly divided into four groups: group I (normal group), group II (model group), group III (Dan-Ning treatment group) and group IV (Dan Shi Jing treatment group). We respectively determined the rates of stone-formed tested bile acid (BA), Monoconjugated bilirubine (MCB), examined the ultrastucture and histochemical changes of the liver using AB-PAS and HID-AB stain, and analyzed the expression of B-UGT and cholesteral 7α-hdroxylase mRNA using reverse transcript polymerase chain reaction (RT-PCR) in each group. Results: compared with group II, the rates of stone-formed, MCB of bile were significantly lowered, the expression of B-UGT and cholesterol 7α-hdroxylase mRNA were up-regulated and the fatty and cholestatic changes of hepatocytes were remarkably alleviated in both group III and group IV.. Conclusion: the therapy of DSPB can up-regulate the expression of the gallstone-related genes B-UGT and cholesterol 7α-hdroxylase mRNA, maintain the stable metabolisms of both bilirubin and cholesterol and decrease the bile lithogenicity. The therapy is likely to be an available treatment of choice in the management of gallstone disease. .

在疏肝利胆法从肝细胞枢纽环节防治胆色素结石基础上，再深入研究其分子机理，玫偷鞍滓持码嗍蟮ㄉ亟崾Ｐ停芯渴韪卫ㄖ幸┒愿卧唳拢璘GT及其MRNA、胆固醇7α－羟化酶MRNA的调控作用。初步在整体上对胆色素结石中医的两个基本证型及其治法与ㄉ亟崾梢蛉龌痉矫娴墓叵档姆肿踊碛薪衔娴牟汀?.