酶层柱材料的插层化学组装及其结构与催化性能研究

Supramolecular pillared structure based on layered host (LDHs) and enzyme molecules was assemblyed through electrostatic adsorption and covalent crosslinking pathway. The influence of intercalation chemical mechanism on the crystal structure, chemical composition, interaction between the host and guest of supramolecular immobilized enzyme was studied. The effect of chemical and geometrical micro-environments on the bio-catalytic performance in the molecular reactor was also investigated. The proposal of the novel concept such as molecular reactor and supramolecular structure provides useful suggestion for the further research. A novel intercalation method called as dissolution-precipitation process was put forward. The intercalation chemistry of enzyme breaks a new path for the application of layered materials and the immobilization of enzyme.

以层状材料为主体、青霉素酰化酶为客体，设计酶层柱材料的结构，研究插层化学组装行为，明确晶体结构、化学组成、化学和几何微环境、酶与层板相互作用对酶机构及性能的影响，建立酶层柱材料的机构模型，探讨其催化机理和动力学以及影响因素。酶插层化学为充分发挥层状材料的结构优势和酶固定化开辟了新的途径，并可创制新型的固定化酶。